Substance P primes the formation of hydrogen peroxide and nitric oxide in human neutrophils

Anja Sterner-Kock, Ruedi K. Braun, Albert Van Der Vliet, Mark D. Schrenzel, Ruth J McDonald, Mahendra B. Kabbur, Philip R Vulliet, Dallas M. Hyde

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Substance P (SP), a neurotransmitter of the central and peripheral nervous system, has been implicated as a mediator of the pulmonary inflammatory response through its stimulatory effects on neutrophils. We investigated the role of SP in priming the production of reactive oxygen species by human neutrophils with the cytochrome c reduction assay and by flow cytometry using the intracellular oxidizable probe dichlorofluorescein. We also investigated SP-induced formation of nitrite and nitrate as an index of nitric oxide (NO) production. Our results indicate that SP primes two distinct pathways with respect to the induction of reactive oxygen species in the human neutrophil: the production of superoxide anion and hydrogen peroxide by the calmodulin-dependent NADPH oxidase, and the generation of NO by a constitutive NO synthase. Preincubation of neutrophils with inhibitors of calmodulin and NO synthase diminished the oxidative response in an additive fashion. These results give insight into distinct signal transduction pathways in the SP-primed neutrophil with respect to the formation of superoxide anion, hydrogen peroxide, and NO.

Original languageEnglish (US)
Pages (from-to)834-840
Number of pages7
JournalJournal of Leukocyte Biology
Volume65
Issue number6
StatePublished - 1999

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Substance P
Hydrogen Peroxide
Nitric Oxide
Neutrophils
Water
Calmodulin
Nitric Oxide Synthase
Superoxides
Reactive Oxygen Species
NADPH Oxidase
Peripheral Nervous System
Nitrites
Cytochromes c
Nitrates
Neurotransmitter Agents
Signal Transduction
Flow Cytometry
Central Nervous System
Lung

Keywords

  • Inflammation
  • Neurotransmitter
  • Oxidative burst

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sterner-Kock, A., Braun, R. K., Van Der Vliet, A., Schrenzel, M. D., McDonald, R. J., Kabbur, M. B., ... Hyde, D. M. (1999). Substance P primes the formation of hydrogen peroxide and nitric oxide in human neutrophils. Journal of Leukocyte Biology, 65(6), 834-840.

Substance P primes the formation of hydrogen peroxide and nitric oxide in human neutrophils. / Sterner-Kock, Anja; Braun, Ruedi K.; Van Der Vliet, Albert; Schrenzel, Mark D.; McDonald, Ruth J; Kabbur, Mahendra B.; Vulliet, Philip R; Hyde, Dallas M.

In: Journal of Leukocyte Biology, Vol. 65, No. 6, 1999, p. 834-840.

Research output: Contribution to journalArticle

Sterner-Kock, A, Braun, RK, Van Der Vliet, A, Schrenzel, MD, McDonald, RJ, Kabbur, MB, Vulliet, PR & Hyde, DM 1999, 'Substance P primes the formation of hydrogen peroxide and nitric oxide in human neutrophils', Journal of Leukocyte Biology, vol. 65, no. 6, pp. 834-840.
Sterner-Kock A, Braun RK, Van Der Vliet A, Schrenzel MD, McDonald RJ, Kabbur MB et al. Substance P primes the formation of hydrogen peroxide and nitric oxide in human neutrophils. Journal of Leukocyte Biology. 1999;65(6):834-840.
Sterner-Kock, Anja ; Braun, Ruedi K. ; Van Der Vliet, Albert ; Schrenzel, Mark D. ; McDonald, Ruth J ; Kabbur, Mahendra B. ; Vulliet, Philip R ; Hyde, Dallas M. / Substance P primes the formation of hydrogen peroxide and nitric oxide in human neutrophils. In: Journal of Leukocyte Biology. 1999 ; Vol. 65, No. 6. pp. 834-840.
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