Subsets of CD8+, CD57+ cells in normal, healthy individuals

Correlations with human cytomegalovirus (HCMV) carrier status, phenotypic and functional analysis

E. C Y Wang, Jean A Wiedeman, P. Perera, J. Fisher, L. K. Borysiewicz

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Two different subsets of CD8+, CD57+ cells have been defined, one expressing high levels (CD8(high)+(CD57+)), the other expressing low levels of surface CD8 (CD8(low)+(CD57+)). Increased numbers of CD8(high)+(CD57+) cells correlated with previous HCMV infection. By three-colour fluorescence analysis, the CD8(high)+(CD57+) population expressed T cell markers such as CD3 and CD5, and most were αβ T cell receptor (αβ TCR)-positive. A significant proportion also expressed CD71 (transferrin receptor) and MHC class II, although little if any CD25 (IL-2R-p55). Some (≥40%) co-expressed CD45RA and CD45RO. The CD8(low)+(CD57+) population expressed classical natural killer (NK) cell markers - CD2, CD16 and CD56. The two subsets were also functionally distinct; CD8(high)+(CD57+) cells suppressed pokeweed mitogen (PWM)-driven, but not phytohaemagglutinin (PHA)-driven proliferation and immunoglobulin production; CD8(low)+(CD57+) cells exhibited NK cytotoxic activity which was not increased by interferon-alpha (IFN-α). Supernatant from cultured CD8(high)+(CD57+) cells suppressed PWM-driven immunoglobulin production, but not proliferation, and this effect was abrogated by physical separation with tissue culture inserts. Thus, a T cell subset expressing activation and memory T cell markers with direct non-specific suppressor activity was present in peripheral blood mononuclear cells (PBMC) of healthy subjects with asymptomatic HCMV infection.

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalClinical and Experimental Immunology
Volume94
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

Cytomegalovirus
Pokeweed Mitogens
Cytomegalovirus Infections
Natural Killer Cells
Immunoglobulins
T-Lymphocytes
Transferrin Receptors
T-Lymphocyte Subsets
Phytohemagglutinins
T-Cell Antigen Receptor
Interferon-alpha
Population
Blood Cells
Healthy Volunteers
Color
Fluorescence

Keywords

  • CD57 cells
  • CD8
  • flow cytometry
  • human cytomegalovirus
  • NK cells
  • T cells

ASJC Scopus subject areas

  • Immunology

Cite this

Subsets of CD8+, CD57+ cells in normal, healthy individuals : Correlations with human cytomegalovirus (HCMV) carrier status, phenotypic and functional analysis. / Wang, E. C Y; Wiedeman, Jean A; Perera, P.; Fisher, J.; Borysiewicz, L. K.

In: Clinical and Experimental Immunology, Vol. 94, No. 2, 1993, p. 297-305.

Research output: Contribution to journalArticle

@article{170fdd6271eb4670b0b4a68669bc5f86,
title = "Subsets of CD8+, CD57+ cells in normal, healthy individuals: Correlations with human cytomegalovirus (HCMV) carrier status, phenotypic and functional analysis",
abstract = "Two different subsets of CD8+, CD57+ cells have been defined, one expressing high levels (CD8(high)+(CD57+)), the other expressing low levels of surface CD8 (CD8(low)+(CD57+)). Increased numbers of CD8(high)+(CD57+) cells correlated with previous HCMV infection. By three-colour fluorescence analysis, the CD8(high)+(CD57+) population expressed T cell markers such as CD3 and CD5, and most were αβ T cell receptor (αβ TCR)-positive. A significant proportion also expressed CD71 (transferrin receptor) and MHC class II, although little if any CD25 (IL-2R-p55). Some (≥40{\%}) co-expressed CD45RA and CD45RO. The CD8(low)+(CD57+) population expressed classical natural killer (NK) cell markers - CD2, CD16 and CD56. The two subsets were also functionally distinct; CD8(high)+(CD57+) cells suppressed pokeweed mitogen (PWM)-driven, but not phytohaemagglutinin (PHA)-driven proliferation and immunoglobulin production; CD8(low)+(CD57+) cells exhibited NK cytotoxic activity which was not increased by interferon-alpha (IFN-α). Supernatant from cultured CD8(high)+(CD57+) cells suppressed PWM-driven immunoglobulin production, but not proliferation, and this effect was abrogated by physical separation with tissue culture inserts. Thus, a T cell subset expressing activation and memory T cell markers with direct non-specific suppressor activity was present in peripheral blood mononuclear cells (PBMC) of healthy subjects with asymptomatic HCMV infection.",
keywords = "CD57 cells, CD8, flow cytometry, human cytomegalovirus, NK cells, T cells",
author = "Wang, {E. C Y} and Wiedeman, {Jean A} and P. Perera and J. Fisher and Borysiewicz, {L. K.}",
year = "1993",
language = "English (US)",
volume = "94",
pages = "297--305",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Subsets of CD8+, CD57+ cells in normal, healthy individuals

T2 - Correlations with human cytomegalovirus (HCMV) carrier status, phenotypic and functional analysis

AU - Wang, E. C Y

AU - Wiedeman, Jean A

AU - Perera, P.

AU - Fisher, J.

AU - Borysiewicz, L. K.

PY - 1993

Y1 - 1993

N2 - Two different subsets of CD8+, CD57+ cells have been defined, one expressing high levels (CD8(high)+(CD57+)), the other expressing low levels of surface CD8 (CD8(low)+(CD57+)). Increased numbers of CD8(high)+(CD57+) cells correlated with previous HCMV infection. By three-colour fluorescence analysis, the CD8(high)+(CD57+) population expressed T cell markers such as CD3 and CD5, and most were αβ T cell receptor (αβ TCR)-positive. A significant proportion also expressed CD71 (transferrin receptor) and MHC class II, although little if any CD25 (IL-2R-p55). Some (≥40%) co-expressed CD45RA and CD45RO. The CD8(low)+(CD57+) population expressed classical natural killer (NK) cell markers - CD2, CD16 and CD56. The two subsets were also functionally distinct; CD8(high)+(CD57+) cells suppressed pokeweed mitogen (PWM)-driven, but not phytohaemagglutinin (PHA)-driven proliferation and immunoglobulin production; CD8(low)+(CD57+) cells exhibited NK cytotoxic activity which was not increased by interferon-alpha (IFN-α). Supernatant from cultured CD8(high)+(CD57+) cells suppressed PWM-driven immunoglobulin production, but not proliferation, and this effect was abrogated by physical separation with tissue culture inserts. Thus, a T cell subset expressing activation and memory T cell markers with direct non-specific suppressor activity was present in peripheral blood mononuclear cells (PBMC) of healthy subjects with asymptomatic HCMV infection.

AB - Two different subsets of CD8+, CD57+ cells have been defined, one expressing high levels (CD8(high)+(CD57+)), the other expressing low levels of surface CD8 (CD8(low)+(CD57+)). Increased numbers of CD8(high)+(CD57+) cells correlated with previous HCMV infection. By three-colour fluorescence analysis, the CD8(high)+(CD57+) population expressed T cell markers such as CD3 and CD5, and most were αβ T cell receptor (αβ TCR)-positive. A significant proportion also expressed CD71 (transferrin receptor) and MHC class II, although little if any CD25 (IL-2R-p55). Some (≥40%) co-expressed CD45RA and CD45RO. The CD8(low)+(CD57+) population expressed classical natural killer (NK) cell markers - CD2, CD16 and CD56. The two subsets were also functionally distinct; CD8(high)+(CD57+) cells suppressed pokeweed mitogen (PWM)-driven, but not phytohaemagglutinin (PHA)-driven proliferation and immunoglobulin production; CD8(low)+(CD57+) cells exhibited NK cytotoxic activity which was not increased by interferon-alpha (IFN-α). Supernatant from cultured CD8(high)+(CD57+) cells suppressed PWM-driven immunoglobulin production, but not proliferation, and this effect was abrogated by physical separation with tissue culture inserts. Thus, a T cell subset expressing activation and memory T cell markers with direct non-specific suppressor activity was present in peripheral blood mononuclear cells (PBMC) of healthy subjects with asymptomatic HCMV infection.

KW - CD57 cells

KW - CD8

KW - flow cytometry

KW - human cytomegalovirus

KW - NK cells

KW - T cells

UR - http://www.scopus.com/inward/record.url?scp=0027441767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027441767&partnerID=8YFLogxK

M3 - Article

VL - 94

SP - 297

EP - 305

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 2

ER -