Subsets of CD8+, CD57+ cells in normal, healthy individuals: Correlations with human cytomegalovirus (HCMV) carrier status, phenotypic and functional analysis

E. C Y Wang, Jean A Wiedeman, P. Perera, J. Fisher, L. K. Borysiewicz

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Two different subsets of CD8+, CD57+ cells have been defined, one expressing high levels (CD8(high)+(CD57+)), the other expressing low levels of surface CD8 (CD8(low)+(CD57+)). Increased numbers of CD8(high)+(CD57+) cells correlated with previous HCMV infection. By three-colour fluorescence analysis, the CD8(high)+(CD57+) population expressed T cell markers such as CD3 and CD5, and most were αβ T cell receptor (αβ TCR)-positive. A significant proportion also expressed CD71 (transferrin receptor) and MHC class II, although little if any CD25 (IL-2R-p55). Some (≥40%) co-expressed CD45RA and CD45RO. The CD8(low)+(CD57+) population expressed classical natural killer (NK) cell markers - CD2, CD16 and CD56. The two subsets were also functionally distinct; CD8(high)+(CD57+) cells suppressed pokeweed mitogen (PWM)-driven, but not phytohaemagglutinin (PHA)-driven proliferation and immunoglobulin production; CD8(low)+(CD57+) cells exhibited NK cytotoxic activity which was not increased by interferon-alpha (IFN-α). Supernatant from cultured CD8(high)+(CD57+) cells suppressed PWM-driven immunoglobulin production, but not proliferation, and this effect was abrogated by physical separation with tissue culture inserts. Thus, a T cell subset expressing activation and memory T cell markers with direct non-specific suppressor activity was present in peripheral blood mononuclear cells (PBMC) of healthy subjects with asymptomatic HCMV infection.

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalClinical and Experimental Immunology
Volume94
Issue number2
StatePublished - 1993
Externally publishedYes

Keywords

  • CD57 cells
  • CD8
  • flow cytometry
  • human cytomegalovirus
  • NK cells
  • T cells

ASJC Scopus subject areas

  • Immunology

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