Subregional, dendritic compartment, and spine subtype specificity in cocaine regulation of dendritic spines in the nucleus accumbens

Dani Dumitriu, Quincey Laplant, Yael S. Grossman, Caroline Dias, William G. Janssen, Scott J. Russo, John Morrison, Eric J. Nestler

Research output: Contribution to journalArticle

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Abstract

Numerous studies have found that chronic cocaine increases dendritic spine density of medium spiny neuronsinthe nucleus accumbens (NAc). Here, we used single-cell microinjections and advanced 3D imaging and analysis techniques to extend these findings in several important ways: byassessing cocaine regulation of dendritic spinesinthe core versusshell sub regions of NAcinthe mouse, over abroad time course (4 h, 24 h, or 28 d) of withdrawal from chronic cocaine, and with a particular focus on proximal versus distal dendrites. Our data demonstrate subregion-specific, and in some cases opposite, regulation of spines by cocaine on proximal but not distal dendrites. Notably, all observed density changes were attributable to selective regulation of thin spines. At 4 h after injection, the proximal spine density is unchanged in the core but significantly increased in the shell. At 24 h, the density of proximal dendritic spines is reduced in the core but increased in the shell. Such down regulation of thin spines in the core persists through 28 d of withdrawal, whereas the spine density in the shell returns to baseline levels. Consistent with previous results, dendritic tips exhibited up regulation of dendritic spines after 24 h of withdrawal, an effect localized to the shell. The divergence in regulation of proximal spine density in NAc core versus shell by cocaine correlates with recently reported electrophysiological data from a similar drug administration regimen and might represent a key mediator of changes in the reward circuit that drive aspects of addiction.

Original languageEnglish (US)
Pages (from-to)6957-6966
Number of pages10
JournalJournal of Neuroscience
Volume32
Issue number20
DOIs
StatePublished - May 16 2012
Externally publishedYes

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Dendritic Spines
Nucleus Accumbens
Cocaine
Spine
Dendrites
Microinjections
Reward
Up-Regulation
Down-Regulation
Injections
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dumitriu, D., Laplant, Q., Grossman, Y. S., Dias, C., Janssen, W. G., Russo, S. J., ... Nestler, E. J. (2012). Subregional, dendritic compartment, and spine subtype specificity in cocaine regulation of dendritic spines in the nucleus accumbens. Journal of Neuroscience, 32(20), 6957-6966. https://doi.org/10.1523/JNEUROSCI.5718-11.2012

Subregional, dendritic compartment, and spine subtype specificity in cocaine regulation of dendritic spines in the nucleus accumbens. / Dumitriu, Dani; Laplant, Quincey; Grossman, Yael S.; Dias, Caroline; Janssen, William G.; Russo, Scott J.; Morrison, John; Nestler, Eric J.

In: Journal of Neuroscience, Vol. 32, No. 20, 16.05.2012, p. 6957-6966.

Research output: Contribution to journalArticle

Dumitriu, Dani ; Laplant, Quincey ; Grossman, Yael S. ; Dias, Caroline ; Janssen, William G. ; Russo, Scott J. ; Morrison, John ; Nestler, Eric J. / Subregional, dendritic compartment, and spine subtype specificity in cocaine regulation of dendritic spines in the nucleus accumbens. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 20. pp. 6957-6966.
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