Submandibular gland biopsy for the diagnosis of parkinson disease

Thomas G. Beach, Charles H. Adler, Brittany Dugger, Geidy Serrano, Jose Hidalgo, Jonette Henry-Watson, Holly A. Shill, Lucia I. Sue, Marwan N. Sabbagh, Haruhiko Akiyama

Research output: Contribution to journalArticle

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Abstract

The clinical diagnosis of Parkinson disease (PD) is incorrect in 30% or more of subjects particularly at the time of symptom onset. Because Lewy-type α-synucleinopathy is present in the submandibular glands of PD patients, we assessed the feasibility of submandibular gland biopsy for diagnosing PD. We performed immunohistochemical staining for Lewy-type α-synucleinopathy in sections of large segments (simulating open biopsy) and needle cores of submandibular glands from 128 autopsied and neuropathologically classified subjects, including 28 PD, 5 incidental Lewy body disease, 5 progressive supranuclear palsy (3 with concurrent PD), 3 corticobasal degeneration, 2 multiple system atrophy, 22 Alzheimer disease with Lewy bodies, 16 Alzheimer disease without Lewy bodies, and 50 normal elderly. Immunoreactive nerve fibers were present in large submandibular gland sections of all 28 PD subjects (including 3 that also had progressive supranuclear palsy); 3 Alzheimer disease with Lewy bodies subjects were also positive, but none of the other subjects were positive. Cores from frozen submandibular glands taken with 18-gauge needles (total length, 15-38 mm; between 10 and 118 sections per subject examined) were positive for Lewy-type α-synucleinopathy in 17 of 19 PD patients. These results suggest that biopsy of the submandibular gland may be a feasible means of improving PD clinical diagnostic accuracy. This would be particularly advantageous for subject selection in early-stage clinical trials for invasive therapies or for verifying other biomarker studies.

Original languageEnglish (US)
Pages (from-to)130-136
Number of pages7
JournalJournal of Neuropathology and Experimental Neurology
Volume72
Issue number2
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

Fingerprint

Submandibular Gland
Parkinson Disease
Biopsy
Lewy Body Disease
Submandibular Gland Diseases
Large-Core Needle Biopsy
Progressive Supranuclear Palsy
Multiple System Atrophy
Lewy Bodies
Nerve Fibers
Patient Selection
Needles
Alzheimer Disease
Biomarkers
Clinical Trials
Staining and Labeling

Keywords

  • α-Synuclein
  • Biomarker
  • Clinical trial
  • Deep brain stimulation
  • Gene therapy
  • Lewy body
  • Parkinson disease
  • Surgery
  • Transplantation.

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Beach, T. G., Adler, C. H., Dugger, B., Serrano, G., Hidalgo, J., Henry-Watson, J., ... Akiyama, H. (2013). Submandibular gland biopsy for the diagnosis of parkinson disease. Journal of Neuropathology and Experimental Neurology, 72(2), 130-136. https://doi.org/10.1097/NEN.0b013e3182805c72

Submandibular gland biopsy for the diagnosis of parkinson disease. / Beach, Thomas G.; Adler, Charles H.; Dugger, Brittany; Serrano, Geidy; Hidalgo, Jose; Henry-Watson, Jonette; Shill, Holly A.; Sue, Lucia I.; Sabbagh, Marwan N.; Akiyama, Haruhiko.

In: Journal of Neuropathology and Experimental Neurology, Vol. 72, No. 2, 01.02.2013, p. 130-136.

Research output: Contribution to journalArticle

Beach, TG, Adler, CH, Dugger, B, Serrano, G, Hidalgo, J, Henry-Watson, J, Shill, HA, Sue, LI, Sabbagh, MN & Akiyama, H 2013, 'Submandibular gland biopsy for the diagnosis of parkinson disease', Journal of Neuropathology and Experimental Neurology, vol. 72, no. 2, pp. 130-136. https://doi.org/10.1097/NEN.0b013e3182805c72
Beach, Thomas G. ; Adler, Charles H. ; Dugger, Brittany ; Serrano, Geidy ; Hidalgo, Jose ; Henry-Watson, Jonette ; Shill, Holly A. ; Sue, Lucia I. ; Sabbagh, Marwan N. ; Akiyama, Haruhiko. / Submandibular gland biopsy for the diagnosis of parkinson disease. In: Journal of Neuropathology and Experimental Neurology. 2013 ; Vol. 72, No. 2. pp. 130-136.
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