Abstract
Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 356-368 |
| Number of pages | 13 |
| Journal | Virology |
| Volume | 135 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1984 |
Fingerprint
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus. / Privalsky, Martin L.; Michael Bishop, J.
In: Virology, Vol. 135, No. 2, 1984, p. 356-368.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus
AU - Privalsky, Martin L.
AU - Michael Bishop, J.
PY - 1984
Y1 - 1984
N2 - Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.
AB - Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.
UR - http://www.scopus.com/inward/record.url?scp=0021166294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021166294&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(84)90192-2
DO - 10.1016/0042-6822(84)90192-2
M3 - Article
C2 - 6330978
AN - SCOPUS:0021166294
VL - 135
SP - 356
EP - 368
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -