Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus

Martin L. Privalsky, J. Michael Bishop

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.

Original languageEnglish (US)
Pages (from-to)356-368
Number of pages13
JournalVirology
Volume135
Issue number2
DOIs
StatePublished - 1984

Fingerprint

Alpharetrovirus
Oncogenes
Protein Kinases
Erythroid Cells
Membranes
Retroviridae
Amino Acid Sequence
Glycoproteins
Membrane Proteins
Proteins
Fibroblasts
Cell Membrane
Genome
IgA receptor

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus. / Privalsky, Martin L.; Michael Bishop, J.

In: Virology, Vol. 135, No. 2, 1984, p. 356-368.

Research output: Contribution to journalArticle

Privalsky, Martin L. ; Michael Bishop, J. / Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus. In: Virology. 1984 ; Vol. 135, No. 2. pp. 356-368.
@article{446321ad9a1c4b6b9671b475261986a0,
title = "Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus",
abstract = "Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.",
author = "Privalsky, {Martin L.} and {Michael Bishop}, J.",
year = "1984",
doi = "10.1016/0042-6822(84)90192-2",
language = "English (US)",
volume = "135",
pages = "356--368",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus

AU - Privalsky, Martin L.

AU - Michael Bishop, J.

PY - 1984

Y1 - 1984

N2 - Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.

AB - Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.

UR - http://www.scopus.com/inward/record.url?scp=0021166294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021166294&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(84)90192-2

DO - 10.1016/0042-6822(84)90192-2

M3 - Article

VL - 135

SP - 356

EP - 368

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -