Subcellular localization of the v-erb-B protein, the product of a transforming gene of avian erythroblastosis virus

Martin L. Privalsky, J. Michael Bishop

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Avian erythroblastosis virus (AEV) is an oncogenic retrovirus capable of transforming both fibroblasts and immature erythroid cells. The v-erb-B locus within the AEV genome encodes a glycosylated protein, expression of which is required for oncogenic transformation of either cell type. Subcellular localization of the v-erb-B glycoprotein in AEV-transformed cells is reported here. Results indicate that the v-erb-B protein is synthesized on dense membrane fractions and appears to possess the properties of an integral membrane protein. The bulk of the v-erb-B protein remains with dense membranes after synthesis, although a small quantity may slowly become associated with the plasma membrane. The biogenesis and subcellular location of the v-erb-B protein are thus quite different from those of the transforming proteins that display protein kinase activity. These differences are especially provocative because the amino acid sequences of the v-erb-B protein and the protein kinases are closely related to one another.

Original languageEnglish (US)
Pages (from-to)356-368
Number of pages13
JournalVirology
Volume135
Issue number2
DOIs
StatePublished - 1984

    Fingerprint

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this