Study on the selective restriction of mass transport during isoelectric focusing

Cyrill Naydenov, Evgeni Kirazov, Simeon Boyd, Vanio Mitev

Research output: Contribution to journalArticle

Abstract

We separated the electrode solutions by Nafion® membranes, which selectively restrict the processes of migration and diffusion in the whole electrophoretic system. Under these newly developed conditions the ampholyte pH gradient is strongly affected, behaving as a carrier with a very low ionic strength, in which the magnitude of the current flowing through the system rapidly decreases. Thus the isoelectric focusing can be carried out closer to steady state, obtaining sharp protein separation for a considerably shorter period of time.

Original languageEnglish (US)
Pages (from-to)961-968
Number of pages8
JournalComptes Rendus de L'Academie Bulgare des Sciences
Volume65
Issue number7
Publication statusPublished - 2012

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Keywords

  • Electrode current
  • Ionic strength
  • Isoelectric focusing
  • Nafion® effect

ASJC Scopus subject areas

  • General

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