Abstract
The solvent effect on the chlorophyll a (chl a) laser induced fluorescence at different pigment concentrations is reported, when nitrogen pulsed lasers are used as pumping sources. It comes out that the fluorescence radiation spectral distribution is strongly dependent on the pigment concentration and seems to be associated with chl a dimer formation. The self-absorption process was observed as function of pigment concentration and sampling set-up. Under intense nitrogen laser irradiation, gain, linewidth narrowing and lifetime shortening were observed as a result of stimulated emission of radiation which is characteristic to the (0,0) vibronic region of the chl a fluorescence band. Since the number of excitation photons exceeds with about two orders of magnitude the number of chl a molecules in the irradiated volume, a high density of excited singlet states is produced; possible mechanisms for energy losses that prevent significant increasing of S1 population, besides fluorescence, lasing emission and intersystem crossing, are absorption from the S1 excited state and exciplex formation.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | Valentin I. Vlad |
| Pages | 344-346 |
| Number of pages | 3 |
| Volume | 2461 |
| State | Published - 1995 |
| Externally published | Yes |
| Event | ROMOPTO '94: 4th Conference in Optics - Bucharest, Rom Duration: Sep 5 1994 → Sep 8 1994 |
Other
| Other | ROMOPTO '94: 4th Conference in Optics |
|---|---|
| City | Bucharest, Rom |
| Period | 9/5/94 → 9/8/94 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Study of chlorophyll a (in vitro) flourescence induced by nitrogen-pulsed laser radiation. / Wachsmann-Hogiu, Sebastian; Pascu, Mihail L.; Tugulea, Laura.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / Valentin I. Vlad. Vol. 2461 1995. p. 344-346.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Study of chlorophyll a (in vitro) flourescence induced by nitrogen-pulsed laser radiation
AU - Wachsmann-Hogiu, Sebastian
AU - Pascu, Mihail L.
AU - Tugulea, Laura
PY - 1995
Y1 - 1995
N2 - The solvent effect on the chlorophyll a (chl a) laser induced fluorescence at different pigment concentrations is reported, when nitrogen pulsed lasers are used as pumping sources. It comes out that the fluorescence radiation spectral distribution is strongly dependent on the pigment concentration and seems to be associated with chl a dimer formation. The self-absorption process was observed as function of pigment concentration and sampling set-up. Under intense nitrogen laser irradiation, gain, linewidth narrowing and lifetime shortening were observed as a result of stimulated emission of radiation which is characteristic to the (0,0) vibronic region of the chl a fluorescence band. Since the number of excitation photons exceeds with about two orders of magnitude the number of chl a molecules in the irradiated volume, a high density of excited singlet states is produced; possible mechanisms for energy losses that prevent significant increasing of S1 population, besides fluorescence, lasing emission and intersystem crossing, are absorption from the S1 excited state and exciplex formation.
AB - The solvent effect on the chlorophyll a (chl a) laser induced fluorescence at different pigment concentrations is reported, when nitrogen pulsed lasers are used as pumping sources. It comes out that the fluorescence radiation spectral distribution is strongly dependent on the pigment concentration and seems to be associated with chl a dimer formation. The self-absorption process was observed as function of pigment concentration and sampling set-up. Under intense nitrogen laser irradiation, gain, linewidth narrowing and lifetime shortening were observed as a result of stimulated emission of radiation which is characteristic to the (0,0) vibronic region of the chl a fluorescence band. Since the number of excitation photons exceeds with about two orders of magnitude the number of chl a molecules in the irradiated volume, a high density of excited singlet states is produced; possible mechanisms for energy losses that prevent significant increasing of S1 population, besides fluorescence, lasing emission and intersystem crossing, are absorption from the S1 excited state and exciplex formation.
UR - http://www.scopus.com/inward/record.url?scp=0029238331&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029238331&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0029238331
SN - 0819418137
SN - 9780819418135
VL - 2461
SP - 344
EP - 346
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Vlad, Valentin I.
ER -