Study of chlorophyll a (in vitro) flourescence induced by nitrogen-pulsed laser radiation

Sebastian Wachsmann-Hogiu, Mihail L. Pascu, Laura Tugulea

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The solvent effect on the chlorophyll a (chl a) laser induced fluorescence at different pigment concentrations is reported, when nitrogen pulsed lasers are used as pumping sources. It comes out that the fluorescence radiation spectral distribution is strongly dependent on the pigment concentration and seems to be associated with chl a dimer formation. The self-absorption process was observed as function of pigment concentration and sampling set-up. Under intense nitrogen laser irradiation, gain, linewidth narrowing and lifetime shortening were observed as a result of stimulated emission of radiation which is characteristic to the (0,0) vibronic region of the chl a fluorescence band. Since the number of excitation photons exceeds with about two orders of magnitude the number of chl a molecules in the irradiated volume, a high density of excited singlet states is produced; possible mechanisms for energy losses that prevent significant increasing of S1 population, besides fluorescence, lasing emission and intersystem crossing, are absorption from the S1 excited state and exciplex formation.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsValentin I. Vlad
Number of pages3
StatePublished - 1995
Externally publishedYes
EventROMOPTO '94: 4th Conference in Optics - Bucharest, Rom
Duration: Sep 5 1994Sep 8 1994


OtherROMOPTO '94: 4th Conference in Optics
CityBucharest, Rom

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics


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