Studies on human immunodeficiency virus-induced cytopathic effects: use of human rhabdomyosarcoma (RD) cells

A. Srinivasan, C. S. Goldsmith, D. York, R. Anand, Paul A Luciw, G. Schochetman, E. Palmer, C. Bohan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A full-length molecular clone of human immunodeficiency virus (HIV) proviral DNA was transferred to human rhabdomyosarcoma (RD) cells by gene transfer method. RD cells released infectious virus within 12 hours after transfection and the viral particles present in the culture medium could be quantitated by monitoring reverse transcriptase activity. Chronic low level viral producer cell lines of RD were also established. Southern hybridization analysis revealed the presence of HIV sequences in transfected RD cells. Electron microscopic studies of the transfected cell revealed intracellular budding of HIV and also showed structural abnormalities such as giant cell phenotype and vacuolation. These features qualify RD cells as a useful system for studying the regulation and cytopathic effects of HIV.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalArchives of Virology
Volume99
Issue number1-2
DOIs
StatePublished - Mar 1988

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

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