Several strains of Cryptococcus neoformans were found to produce small amounts of ethanol (C2H5OH) in glucose-containing media as detected by an enzymatic (alcohol dehydrogenase) and a gas chromatographic method. In glucose yeast extract broth, in which Saccharomyces cerevisiae and Candida albicans produced greater than half the theoretical yield, C. neoformans produced less than 1% of the theoretical yield. Detection of ethanol in the spinal fluid for diagnosis of cryptococcal meningitis appears to be of limited value, unless there is routine determination of ethanol by one of the 2 methods applied, and because of differing yields of ethanol by differing strains.
ASJC Scopus subject areas
- Infectious Diseases