Studies on a murine model for evaluation of virulence of Streptococcus suis capsular type 2 isolates

M. Beaudoin, Robert Higgins, J. Harel, M. Gottschalk

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Five different parameters, time of incubation of the culture, type of culture medium, inoculum, strain of inbred mice, and age of mice, were tested using the LD50 technique to standardize a murine model for the evaluation of the virulence of Streptococcus suis capsular type 2 isolates. A model using 28 day-old mice belonging to CF1 strain appeard to give the best results. The inoculum size was the parameter most influencing the 50% lethal dose obtained with mice. Inoculation with 1-ml volume of a bacterial suspension instead of 0.1 or 0.5 ml decreased the LD50. The standardized model was used to evaluate the virulence of some isolates of known pathogenicity for pigs. The minimum lethal dose was used in the model and it appeared that the virulence of Streptococcus suis capsular type 2 isolates can be measured from highly virulent to totally avirulent.

Original languageEnglish (US)
Pages (from-to)111-116
Number of pages6
JournalFEMS Microbiology Letters
Volume99
Issue number2-3
DOIs
StatePublished - Dec 1 1992
Externally publishedYes

Keywords

  • LD
  • Murine model
  • Streptococcus suis
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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