Studies of Rhodamine-123: Effect on rat prostate cancer and human prostate cancer cells in vitro

J. A. Arcadi, K. S. Narayan, G. Techy, C. P. Ng, R. M G Saroufeem, L. W. Jones, T. V. Shankey

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect of the lipophilic, cationic dye, Rhodamine-123 (Rh-123), on prostate cancer in rats, and on three tumor cell lines in vitro is reported here. The general toxicity of Rh-123 in mice has been found to be minimal. Lobund-Wistar (L-W) rats with the autochthonous prostate cancer of Pollard were treated for six doses with Rh-123 at a dose of 15 mg/kg subcutaneously every other day. Microscopic examination of the tumors revealed cellular and acinar destruction. The effectiveness of Rh-123 as a cytotoxic agent was tested by clonogenic and viability assays in vitro with three human prostate cancer cell lines. Severe (60-95%) growth inhibition was observed following Rh-123 exposure for 2-5 days at doses as low as 1.6 μg/ml in all three prostate cancer cell lines.

Original languageEnglish (US)
Pages (from-to)86-93
Number of pages8
JournalJournal of Surgical Oncology
Volume59
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Rhodamine 123
Prostatic Neoplasms
Cell Line
Cytotoxins
Tumor Cell Line
Wistar Rats
Coloring Agents
In Vitro Techniques
Growth
Neoplasms

Keywords

  • autochthonous rat prostate tumor
  • chemotherapy
  • mitochondrial toxin
  • prostate cancer
  • Rhodamine-123

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Arcadi, J. A., Narayan, K. S., Techy, G., Ng, C. P., Saroufeem, R. M. G., Jones, L. W., & Shankey, T. V. (1995). Studies of Rhodamine-123: Effect on rat prostate cancer and human prostate cancer cells in vitro. Journal of Surgical Oncology, 59(2), 86-93.

Studies of Rhodamine-123 : Effect on rat prostate cancer and human prostate cancer cells in vitro. / Arcadi, J. A.; Narayan, K. S.; Techy, G.; Ng, C. P.; Saroufeem, R. M G; Jones, L. W.; Shankey, T. V.

In: Journal of Surgical Oncology, Vol. 59, No. 2, 1995, p. 86-93.

Research output: Contribution to journalArticle

Arcadi, JA, Narayan, KS, Techy, G, Ng, CP, Saroufeem, RMG, Jones, LW & Shankey, TV 1995, 'Studies of Rhodamine-123: Effect on rat prostate cancer and human prostate cancer cells in vitro', Journal of Surgical Oncology, vol. 59, no. 2, pp. 86-93.
Arcadi JA, Narayan KS, Techy G, Ng CP, Saroufeem RMG, Jones LW et al. Studies of Rhodamine-123: Effect on rat prostate cancer and human prostate cancer cells in vitro. Journal of Surgical Oncology. 1995;59(2):86-93.
Arcadi, J. A. ; Narayan, K. S. ; Techy, G. ; Ng, C. P. ; Saroufeem, R. M G ; Jones, L. W. ; Shankey, T. V. / Studies of Rhodamine-123 : Effect on rat prostate cancer and human prostate cancer cells in vitro. In: Journal of Surgical Oncology. 1995 ; Vol. 59, No. 2. pp. 86-93.
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