Studies of DNA packaging into the heads of bacteriophage lambda

Michael Syvanen, Jerry Yin

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

During the assembly of bacteriophage λ heads, a head-like, DNA-free structure called petite λ, is first constructed. Into this, λ DNA is then packaged. In this paper we examine early interactions between λ DNA and petite λ in a cell-free system. The two major findings of this paper are: (1) when seen through the electron microscope, an early petite λ-λ DNA complex appears with the circular petite λ having the DNA crossing through its center. These resemble a bead on a string or the Greek letter φ (hence they are called φ structures). The λ A protein is required in the formation of φ structures. Also, φ structures can be found in bacteria infected with phage λ. (2) The polyamine putrescine is required for phage head assembly. An earlier reported requirement for spermidine can be replaced by the addition of putrescine. Polyamine is required in the DNA packaging reaction after the packaging has begun.

Original languageEnglish (US)
Pages (from-to)333-346
Number of pages14
JournalJournal of Molecular Biology
Volume126
Issue number3
DOIs
StatePublished - Dec 15 1978
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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