Studies of congenitally immunologically mutant New Zealand mice. VIII. Cell-surface phenotype of spleen cells of X(id) and nude mice

R. R. Skelly, K. R. Bray, M. Eric Gershwin, F. Dumont, A. Ahmed

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Abstract

Surface immunoglobulin on spleen cells from NZB and NZB/W mice and congenic mice bearing the nude or X-linked immune defective (X(id)) gene was examined by flow microfluorometry with regard to both the frequency of positive cells and density expressed on the cell. These data indicate that although the frequency of unseparated sIg+ B lymphocytes is equivalent among all of these groups of mice, the densities of sIgM and sIgD are different. Spleen cells from these mice were also separated by free-flow electrophoresis and analyzed in a similar manner. This analysis demonstrated the absence of a subpopulation of B lymphocytes with a low electrophoretic mobility and low expression of sIgM. These studies suggest that maturational and/or activation states of the B cells in mice bearing the X(id) or nude genes are different from those seen in the parent strains of mice. Such alterations in cell-surface antigens correlate with the differences in the natural history of immunopathology of the autoimmune disease in these congenic colonies of New Zealand mice.

Original languageEnglish (US)
Pages (from-to)221-229
Number of pages9
JournalJournal of Immunogenetics
Volume10
Issue number3
StatePublished - 1983

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New Zealand
Nude Mice
Spleen
Phenotype
B-Lymphocytes
Inbred NZB Mouse
Congenic Mice
B-Cell Antigen Receptors
Surface Antigens
Natural History
Genes
Autoimmune Diseases
Electrophoresis
Flow Cytometry
Cell Count

ASJC Scopus subject areas

  • Immunology
  • Genetics

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Studies of congenitally immunologically mutant New Zealand mice. VIII. Cell-surface phenotype of spleen cells of X(id) and nude mice. / Skelly, R. R.; Bray, K. R.; Gershwin, M. Eric; Dumont, F.; Ahmed, A.

In: Journal of Immunogenetics, Vol. 10, No. 3, 1983, p. 221-229.

Research output: Contribution to journalArticle

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AU - Ahmed, A.

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