Structured illumination-based super-resolution optical microscopy for hemato- and cyto-pathology applications

Tieqiao Zhang, Samantha Osborn, Chloe Brandow, Denis M Dwyre, Ralph Green, Stephen Lane, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Structured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of resolution in the z- direction by the same factor of two (to approximately 220 nm), making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy.

Original languageEnglish (US)
Pages (from-to)27-35
Number of pages9
JournalAnalytical Cellular Pathology
Volume36
Issue number1-2
DOIs
StatePublished - 2013

Fingerprint

Lighting
Fluorescence Microscopy
Microscopy
Blood Cells
Abnormal Erythrocytes
Pathology
Light
Three-Dimensional Imaging
Hematology
Erythrocytes
Research
Direction compound

Keywords

  • blood cells
  • structured illumination
  • Super-resolution microscopy

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

Structured illumination-based super-resolution optical microscopy for hemato- and cyto-pathology applications. / Zhang, Tieqiao; Osborn, Samantha; Brandow, Chloe; Dwyre, Denis M; Green, Ralph; Lane, Stephen; Wachsmann-Hogiu, Sebastian.

In: Analytical Cellular Pathology, Vol. 36, No. 1-2, 2013, p. 27-35.

Research output: Contribution to journalArticle

@article{82ec1c4f33fd4d05a6bb6bb870952c86,
title = "Structured illumination-based super-resolution optical microscopy for hemato- and cyto-pathology applications",
abstract = "Structured illumination fluorescence microscopy utilizes interfering light and the moir{\'e} effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of resolution in the z- direction by the same factor of two (to approximately 220 nm), making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy.",
keywords = "blood cells, structured illumination, Super-resolution microscopy",
author = "Tieqiao Zhang and Samantha Osborn and Chloe Brandow and Dwyre, {Denis M} and Ralph Green and Stephen Lane and Sebastian Wachsmann-Hogiu",
year = "2013",
doi = "10.3233/ACP-130075",
language = "English (US)",
volume = "36",
pages = "27--35",
journal = "Analytical Cellular Pathology",
issn = "2210-7177",
publisher = "IOS Press",
number = "1-2",

}

TY - JOUR

T1 - Structured illumination-based super-resolution optical microscopy for hemato- and cyto-pathology applications

AU - Zhang, Tieqiao

AU - Osborn, Samantha

AU - Brandow, Chloe

AU - Dwyre, Denis M

AU - Green, Ralph

AU - Lane, Stephen

AU - Wachsmann-Hogiu, Sebastian

PY - 2013

Y1 - 2013

N2 - Structured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of resolution in the z- direction by the same factor of two (to approximately 220 nm), making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy.

AB - Structured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of resolution in the z- direction by the same factor of two (to approximately 220 nm), making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy.

KW - blood cells

KW - structured illumination

KW - Super-resolution microscopy

UR - http://www.scopus.com/inward/record.url?scp=84878896058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878896058&partnerID=8YFLogxK

U2 - 10.3233/ACP-130075

DO - 10.3233/ACP-130075

M3 - Article

C2 - 23579249

AN - SCOPUS:84878896058

VL - 36

SP - 27

EP - 35

JO - Analytical Cellular Pathology

JF - Analytical Cellular Pathology

SN - 2210-7177

IS - 1-2

ER -