Structure of the OsSERK2 leucine-rich repeat extracellular domain

Ryan McAndrew, Rory N. Pruitt, Shizuo G. Kamita, Jose Henrique Pereira, Dipali Majumdar, Bruce D. Hammock, Paul D. Adams, Pamela C. Ronald

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Somatic embryogenesis receptor kinases (SERKs) are leucine-rich repeat (LRR)-containing integral membrane receptors that are involved in the regulation of development and immune responses in plants. It has recently been shown that rice SERK2 (OsSERK2) is essential for XA21-mediated resistance to the pathogen Xanthomonas oryzae pv. oryzae. OsSERK2 is also required for the BRI1-mediated, FLS2-mediated and EFR-mediated responses to brassinosteroids, flagellin and elongation factor Tu (EF-Tu), respectively. Here, crystal structures of the LRR domains of OsSERK2 and a D128N OsSERK2 mutant, expressed as hagfish variable lymphocyte receptor (VLR) fusions, are reported. These structures suggest that the aspartate mutation does not generate any significant conformational change in the protein, but instead leads to an altered interaction with partner receptors.

Original languageEnglish (US)
Pages (from-to)3080-3086
Number of pages7
JournalActa Crystallographica Section D: Biological Crystallography
Volume70
Issue number11
DOIs
StatePublished - Nov 1 2014

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Leucine
Hagfishes
Plant Immunity
Brassinosteroids
Peptide Elongation Factor Tu
Xanthomonas
Flagellin
Aspartic Acid
Embryonic Development
Phosphotransferases
Lymphocytes
Mutation
Membranes
Oryza
Proteins
leucine receptor

Keywords

  • leucine-rich repeats
  • OsSERK2
  • SERKs

ASJC Scopus subject areas

  • Structural Biology

Cite this

McAndrew, R., Pruitt, R. N., Kamita, S. G., Pereira, J. H., Majumdar, D., Hammock, B. D., ... Ronald, P. C. (2014). Structure of the OsSERK2 leucine-rich repeat extracellular domain. Acta Crystallographica Section D: Biological Crystallography, 70(11), 3080-3086. https://doi.org/10.1107/S1399004714021178

Structure of the OsSERK2 leucine-rich repeat extracellular domain. / McAndrew, Ryan; Pruitt, Rory N.; Kamita, Shizuo G.; Pereira, Jose Henrique; Majumdar, Dipali; Hammock, Bruce D.; Adams, Paul D.; Ronald, Pamela C.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 70, No. 11, 01.11.2014, p. 3080-3086.

Research output: Contribution to journalArticle

McAndrew, R, Pruitt, RN, Kamita, SG, Pereira, JH, Majumdar, D, Hammock, BD, Adams, PD & Ronald, PC 2014, 'Structure of the OsSERK2 leucine-rich repeat extracellular domain', Acta Crystallographica Section D: Biological Crystallography, vol. 70, no. 11, pp. 3080-3086. https://doi.org/10.1107/S1399004714021178
McAndrew, Ryan ; Pruitt, Rory N. ; Kamita, Shizuo G. ; Pereira, Jose Henrique ; Majumdar, Dipali ; Hammock, Bruce D. ; Adams, Paul D. ; Ronald, Pamela C. / Structure of the OsSERK2 leucine-rich repeat extracellular domain. In: Acta Crystallographica Section D: Biological Crystallography. 2014 ; Vol. 70, No. 11. pp. 3080-3086.
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