Structure of the mouse mammary tumor virus

polypeptides and glycoproteins

Y. A. Teramoto, M. J. Puentes, L. J T Young, Robert Cardiff

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The polypeptide and glycoprotein compositions of the mouse mammary tumor virus virion from primary monolayer cultures of BALB/cfC3H mouse mammary tumor cells were studied by polyacrylamide gel electrophoresis by using internal and external labeling and Coomassie blue and periodic acid Schiff (PAS) staining. 12 polypeptides were reproducibly resolved by the combined methods. 5 major polypeptides were demonstrable with estimated molecular weights of 52,000, 36,000, 28,000, 14,000, and 10,000. 7 minor polypeptides were also consistently detected and had estimated molecular weights of 70,000, 60,000, 46,000, 38,000, 30,000, 22,000, and 17,000. Carbohydrate was associated with 5 of these polypeptides as measured by PAS stain or [3H]glucosamine labeling, or both. These glycoproteins had estimated molecular weights of 70,000, 60,000, 52,000, 36,000 and 10,000. The majority of the PAS stain and glucosamine was found in the 52,000 and 36,000 dalton peaks.

Original languageEnglish (US)
Pages (from-to)411-418
Number of pages8
JournalJournal of Virology
Volume13
Issue number2
StatePublished - Jan 1 1974

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Mouse mammary tumor virus
glycoproteins
polypeptides
Glycoproteins
Periodic Acid
Peptides
Molecular Weight
glucosamine
Glucosamine
molecular weight
dyes
acids
Coloring Agents
mammary neoplasms (animal)
virion
Virion
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
Carbohydrates
Staining and Labeling

ASJC Scopus subject areas

  • Immunology

Cite this

Teramoto, Y. A., Puentes, M. J., Young, L. J. T., & Cardiff, R. (1974). Structure of the mouse mammary tumor virus: polypeptides and glycoproteins. Journal of Virology, 13(2), 411-418.

Structure of the mouse mammary tumor virus : polypeptides and glycoproteins. / Teramoto, Y. A.; Puentes, M. J.; Young, L. J T; Cardiff, Robert.

In: Journal of Virology, Vol. 13, No. 2, 01.01.1974, p. 411-418.

Research output: Contribution to journalArticle

Teramoto, YA, Puentes, MJ, Young, LJT & Cardiff, R 1974, 'Structure of the mouse mammary tumor virus: polypeptides and glycoproteins', Journal of Virology, vol. 13, no. 2, pp. 411-418.
Teramoto, Y. A. ; Puentes, M. J. ; Young, L. J T ; Cardiff, Robert. / Structure of the mouse mammary tumor virus : polypeptides and glycoproteins. In: Journal of Virology. 1974 ; Vol. 13, No. 2. pp. 411-418.
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