TY - JOUR
T1 - Structure of the mouse mammary tumor virus
T2 - polypeptides and glycoproteins
AU - Teramoto, Y. A.
AU - Puentes, M. J.
AU - Young, L. J T
AU - Cardiff, R. D.
PY - 1974/1/1
Y1 - 1974/1/1
N2 - The polypeptide and glycoprotein compositions of the mouse mammary tumor virus virion from primary monolayer cultures of BALB/cfC3H mouse mammary tumor cells were studied by polyacrylamide gel electrophoresis by using internal and external labeling and Coomassie blue and periodic acid Schiff (PAS) staining. 12 polypeptides were reproducibly resolved by the combined methods. 5 major polypeptides were demonstrable with estimated molecular weights of 52,000, 36,000, 28,000, 14,000, and 10,000. 7 minor polypeptides were also consistently detected and had estimated molecular weights of 70,000, 60,000, 46,000, 38,000, 30,000, 22,000, and 17,000. Carbohydrate was associated with 5 of these polypeptides as measured by PAS stain or [3H]glucosamine labeling, or both. These glycoproteins had estimated molecular weights of 70,000, 60,000, 52,000, 36,000 and 10,000. The majority of the PAS stain and glucosamine was found in the 52,000 and 36,000 dalton peaks.
AB - The polypeptide and glycoprotein compositions of the mouse mammary tumor virus virion from primary monolayer cultures of BALB/cfC3H mouse mammary tumor cells were studied by polyacrylamide gel electrophoresis by using internal and external labeling and Coomassie blue and periodic acid Schiff (PAS) staining. 12 polypeptides were reproducibly resolved by the combined methods. 5 major polypeptides were demonstrable with estimated molecular weights of 52,000, 36,000, 28,000, 14,000, and 10,000. 7 minor polypeptides were also consistently detected and had estimated molecular weights of 70,000, 60,000, 46,000, 38,000, 30,000, 22,000, and 17,000. Carbohydrate was associated with 5 of these polypeptides as measured by PAS stain or [3H]glucosamine labeling, or both. These glycoproteins had estimated molecular weights of 70,000, 60,000, 52,000, 36,000 and 10,000. The majority of the PAS stain and glucosamine was found in the 52,000 and 36,000 dalton peaks.
UR - http://www.scopus.com/inward/record.url?scp=0015977902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015977902&partnerID=8YFLogxK
M3 - Article
C2 - 4359300
AN - SCOPUS:0015977902
VL - 13
SP - 411
EP - 418
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 2
ER -