Structure of the mouse mammary tumor virus: characterization of bald particles

Robert Cardiff, M. J. Puentes, Y. A J K Teramoto andLund

Research output: Contribution to journalArticle

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Abstract

The polypeptide, antigenic, and morphological structure of the mouse mammary tumor virus was studied following protease digestion of intact virions. Intact, untreated virions (p=1.17 g/ml) had characteristic envelope spikes, five major polypeptides, and were precipitated by antisera against gp52. Two of the major polypeptides with molecular weights of 52,000 (gp52) and 36,000 (gp36), had carbohydrate moieties. Protease treatment resulted in spikeless, 'bald' particles (p=1.14 g/ml), which had altered surface antigenicity and which contained neither gp52 nor gp36. These data indicated that gp52 and gp36 were on the viral envelope. Bald particles retained a 28,000 dalton polypeptide (p28) which was proposed as the major internal polypeptide.

Original languageEnglish (US)
Pages (from-to)1293-1303
Number of pages11
JournalJournal of Virology
Volume14
Issue number5
StatePublished - Dec 1 1974

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Mouse mammary tumor virus
polypeptides
Peptides
virion
Virion
Peptide Hydrolases
proteinases
antiserum
Immune Sera
Digestion
Molecular Weight
digestion
Carbohydrates
molecular weight
carbohydrates

ASJC Scopus subject areas

  • Immunology

Cite this

Cardiff, R., Puentes, M. J., & Teramoto andLund, Y. A. J. K. (1974). Structure of the mouse mammary tumor virus: characterization of bald particles. Journal of Virology, 14(5), 1293-1303.

Structure of the mouse mammary tumor virus : characterization of bald particles. / Cardiff, Robert; Puentes, M. J.; Teramoto andLund, Y. A J K.

In: Journal of Virology, Vol. 14, No. 5, 01.12.1974, p. 1293-1303.

Research output: Contribution to journalArticle

Cardiff, R, Puentes, MJ & Teramoto andLund, YAJK 1974, 'Structure of the mouse mammary tumor virus: characterization of bald particles', Journal of Virology, vol. 14, no. 5, pp. 1293-1303.
Cardiff, Robert ; Puentes, M. J. ; Teramoto andLund, Y. A J K. / Structure of the mouse mammary tumor virus : characterization of bald particles. In: Journal of Virology. 1974 ; Vol. 14, No. 5. pp. 1293-1303.
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