Structure of a rice β-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

Carl R. Simmons, James C. Litts, Ning Huang, Raymond L. Rodriguez

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

A rice β-glucanase gene was sequenced and its expression analyzed at the level of mRNA accumulation. This gene (Gns1) is expressed at relatively low levels in germinating seeds, shoots, leaves, panicles and callus, but it is expressed at higher levels in roots. Expression in the roots appears to be constitutive. Shoots express Gns1 at much higher levels when treated with ethylene, cytokinin, salicylic acid, and fungal elicitors derived from the pathogen Sclerotium oryzae or from the non-pathogen Saccharomyces cereviseae. Shoots also express Gns1 at higher levels in response to wounding. Expression in the shoots is not significantly affected by auxin, gibberellic acid or abscisic acid. The β-glucanase shows 82% amino acid similarity to the barley 1,3;1,4-β-D-glucanases, and from hybridization studies it is the β-glucanase gene in the rice genome closest to the barley 1,3;1,4-β-glucanase EI gene. The mature peptide has a calculated molecular mass of 32 kDa. The gene has a large 3145 bp intron in the codon for the 25th amino acid of the signal peptide. The gene exhibits a very strong codon bias of 99% G+C in the third position of the codon in the mature peptide coding region, but only 61% G+C in the signal peptide region.

Original languageEnglish (US)
Pages (from-to)33-45
Number of pages13
JournalPlant Molecular Biology
Volume18
Issue number1
DOIs
StatePublished - Jan 1992

Fingerprint

Cytokinins
Salicylic Acid
salicylic acid
cytokinins
ethylene
Genes
rice
codons
Codon
shoots
genes
Hordeum
Protein Sorting Signals
signal peptide
Magnaporthe salvinii
barley
peptides
Amino Acids
Abscisic Acid
Saccharomyces

Keywords

  • gene expression
  • gene family
  • glucanase
  • pathogenesis response
  • plant growth regulators
  • stress response

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Structure of a rice β-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors. / Simmons, Carl R.; Litts, James C.; Huang, Ning; Rodriguez, Raymond L.

In: Plant Molecular Biology, Vol. 18, No. 1, 01.1992, p. 33-45.

Research output: Contribution to journalArticle

Simmons, Carl R. ; Litts, James C. ; Huang, Ning ; Rodriguez, Raymond L. / Structure of a rice β-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors. In: Plant Molecular Biology. 1992 ; Vol. 18, No. 1. pp. 33-45.
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