Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Linda C. Johansson; David Arnlund; Gergely Katona; Thomas A. White; Anton Barty; Daniel P. Deponte; Robert L. Shoeman; Cecilia Wickstrand; Amit Sharma; Garth J. Williams; Andrew Aquila; Michael J. Bogan; Carl Caleman; Jan Davidsson; R. Bruce Doak; Matthias Frank; Raimund Fromme; Lorenzo Galli; Ingo Grotjohann; Mark S. Hunter; Stephan Kassemeyer; Richard A. Kirian; Christopher Kupitz; Mengning Liang; Lukas Lomb; Erik Malmerberg; Andrew V. Martin; Marc Messerschmidt; Karol Nass; Lars Redecke; M. Marvin Seibert; Jennie Sjöhamn; Jan Steinbrener; Francesco Stellato; Dingjie Wang; Weixaio Y. Wahlgren; Uwe Weierstall; Sebastian Westenhoff; Nadia A. Zatsepin; Sébastien Boutet; John C.H. Spence; Ilme Schlichting; Henry N. Chapman; Petra Fromme; Richard Neutze

doi:10.1038/ncomms3911

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Linda C. Johansson, David Arnlund, Gergely Katona, Thomas A. White, Anton Barty, Daniel P. Deponte, Robert L. Shoeman, Cecilia Wickstrand, Amit Sharma, Garth J. Williams, Andrew Aquila, Michael J. Bogan, Carl Caleman, Jan Davidsson, R. Bruce Doak, Matthias Frank, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Mark S. HunterStephan Kassemeyer, Richard A. Kirian, Christopher Kupitz, Mengning Liang, Lukas Lomb, Erik Malmerberg, Andrew V. Martin, Marc Messerschmidt, Karol Nass, Lars Redecke, M. Marvin Seibert, Jennie Sjöhamn, Jan Steinbrener, Francesco Stellato, Dingjie Wang, Weixaio Y. Wahlgren, Uwe Weierstall, Sebastian Westenhoff, Nadia A. Zatsepin, Sébastien Boutet, John C.H. Spence, Ilme Schlichting, Henry N. Chapman, Petra Fromme, Richard Neutze

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Original language	English (US)
Article number	2911
Journal	Nature Communications
Volume	4
DOIs	https://doi.org/10.1038/ncomms3911
State	Published - Dec 19 2013
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Johansson, L. C., Arnlund, D., Katona, G., White, T. A., Barty, A., Deponte, D. P., Shoeman, R. L., Wickstrand, C., Sharma, A., Williams, G. J., Aquila, A., Bogan, M. J., Caleman, C., Davidsson, J., Doak, R. B., Frank, M., Fromme, R., Galli, L., Grotjohann, I., ... Neutze, R. (2013). Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. Nature Communications, 4, [2911]. https://doi.org/10.1038/ncomms3911

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. / Johansson, Linda C.; Arnlund, David; Katona, Gergely; White, Thomas A.; Barty, Anton; Deponte, Daniel P.; Shoeman, Robert L.; Wickstrand, Cecilia; Sharma, Amit; Williams, Garth J.; Aquila, Andrew; Bogan, Michael J.; Caleman, Carl; Davidsson, Jan; Doak, R. Bruce; Frank, Matthias; Fromme, Raimund; Galli, Lorenzo; Grotjohann, Ingo; Hunter, Mark S.; Kassemeyer, Stephan; Kirian, Richard A.; Kupitz, Christopher; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik; Martin, Andrew V.; Messerschmidt, Marc; Nass, Karol; Redecke, Lars; Seibert, M. Marvin; Sjöhamn, Jennie; Steinbrener, Jan; Stellato, Francesco; Wang, Dingjie; Wahlgren, Weixaio Y.; Weierstall, Uwe; Westenhoff, Sebastian; Zatsepin, Nadia A.; Boutet, Sébastien; Spence, John C.H.; Schlichting, Ilme; Chapman, Henry N.; Fromme, Petra; Neutze, Richard.

In: Nature Communications, Vol. 4, 2911, 19.12.2013.

Research output: Contribution to journal › Article › peer-review

Johansson, LC, Arnlund, D, Katona, G, White, TA, Barty, A, Deponte, DP, Shoeman, RL, Wickstrand, C, Sharma, A, Williams, GJ, Aquila, A, Bogan, MJ, Caleman, C, Davidsson, J, Doak, RB, Frank, M, Fromme, R, Galli, L, Grotjohann, I, Hunter, MS, Kassemeyer, S, Kirian, RA, Kupitz, C, Liang, M, Lomb, L, Malmerberg, E, Martin, AV, Messerschmidt, M, Nass, K, Redecke, L, Seibert, MM, Sjöhamn, J, Steinbrener, J, Stellato, F, Wang, D, Wahlgren, WY, Weierstall, U, Westenhoff, S, Zatsepin, NA, Boutet, S, Spence, JCH, Schlichting, I, Chapman, HN, Fromme, P & Neutze, R 2013, 'Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography', Nature Communications, vol. 4, 2911. https://doi.org/10.1038/ncomms3911

Johansson, Linda C. ; Arnlund, David ; Katona, Gergely ; White, Thomas A. ; Barty, Anton ; Deponte, Daniel P. ; Shoeman, Robert L. ; Wickstrand, Cecilia ; Sharma, Amit ; Williams, Garth J. ; Aquila, Andrew ; Bogan, Michael J. ; Caleman, Carl ; Davidsson, Jan ; Doak, R. Bruce ; Frank, Matthias ; Fromme, Raimund ; Galli, Lorenzo ; Grotjohann, Ingo ; Hunter, Mark S. ; Kassemeyer, Stephan ; Kirian, Richard A. ; Kupitz, Christopher ; Liang, Mengning ; Lomb, Lukas ; Malmerberg, Erik ; Martin, Andrew V. ; Messerschmidt, Marc ; Nass, Karol ; Redecke, Lars ; Seibert, M. Marvin ; Sjöhamn, Jennie ; Steinbrener, Jan ; Stellato, Francesco ; Wang, Dingjie ; Wahlgren, Weixaio Y. ; Weierstall, Uwe ; Westenhoff, Sebastian ; Zatsepin, Nadia A. ; Boutet, Sébastien ; Spence, John C.H. ; Schlichting, Ilme ; Chapman, Henry N. ; Fromme, Petra ; Neutze, Richard. / Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. In: Nature Communications. 2013 ; Vol. 4.

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abstract = "Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 {\AA} resolution and determine its serial femtosecond crystallography structure to 3.5 {\AA} resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.",

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AU - Barty, Anton

AU - Deponte, Daniel P.

AU - Shoeman, Robert L.

AU - Wickstrand, Cecilia

AU - Sharma, Amit

AU - Williams, Garth J.

AU - Aquila, Andrew

AU - Bogan, Michael J.

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AU - Davidsson, Jan

AU - Doak, R. Bruce

AU - Frank, Matthias

AU - Fromme, Raimund

AU - Galli, Lorenzo

AU - Grotjohann, Ingo

AU - Hunter, Mark S.

AU - Kassemeyer, Stephan

AU - Kirian, Richard A.

AU - Kupitz, Christopher

AU - Liang, Mengning

AU - Lomb, Lukas

AU - Malmerberg, Erik

AU - Martin, Andrew V.

AU - Messerschmidt, Marc

AU - Nass, Karol

AU - Redecke, Lars

AU - Seibert, M. Marvin

AU - Sjöhamn, Jennie

AU - Steinbrener, Jan

AU - Stellato, Francesco

AU - Wang, Dingjie

AU - Wahlgren, Weixaio Y.

AU - Weierstall, Uwe

AU - Westenhoff, Sebastian

AU - Zatsepin, Nadia A.

AU - Boutet, Sébastien

AU - Spence, John C.H.

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AU - Chapman, Henry N.

AU - Fromme, Petra

AU - Neutze, Richard

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Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Abstract

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