Structure of a human rhinovirus-bivalently bound antibody complex: Implications for viral neutralization and antibody flexibility

Thomas J. Smith, Norman H. Olson, R. Holland Cheng, Elaine S. Chase, Timothy S. Baker

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

The structure of a neutralizing immunoglobulin (monoclonal antibody mAb17-IA), bound to human rhinovirus 14 (HRV14), has been determined by cryo-electron microscopy and image reconstruction. The antibody bound bivalently across icosahedral twofold axes of the virus, and there were no detectable conformational changes in the capsid. Thus, bivalently bound IgGs do not appear to cause gross deformations in the capsid. Differences between the electron density of the constant domains of the bound Fab fragment and IgG structures suggested that conformational changes occur about elbow axes upon bivalent attachment as was previously predicted. No significant density was observed for the Fc fragment, which adds further evidence for a high degree of mobility about the hinge region.

Original languageEnglish (US)
Pages (from-to)7015-7018
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number15
StatePublished - Aug 1 1993
Externally publishedYes

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Viral Antibodies
Rhinovirus
Capsid
Immunoglobulin Fc Fragments
Cryoelectron Microscopy
Immunoglobulin Fab Fragments
Computer-Assisted Image Processing
Antibodies
Elbow
Immunoglobulins
Immunoglobulin G
Monoclonal Antibodies
Electrons
Viruses

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Structure of a human rhinovirus-bivalently bound antibody complex : Implications for viral neutralization and antibody flexibility. / Smith, Thomas J.; Olson, Norman H.; Cheng, R. Holland; Chase, Elaine S.; Baker, Timothy S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 15, 01.08.1993, p. 7015-7018.

Research output: Contribution to journalArticle

Smith, Thomas J. ; Olson, Norman H. ; Cheng, R. Holland ; Chase, Elaine S. ; Baker, Timothy S. / Structure of a human rhinovirus-bivalently bound antibody complex : Implications for viral neutralization and antibody flexibility. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 15. pp. 7015-7018.
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