Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase: A crucial enzyme for spores and growing cells of Bacillus species

Masatoshi Nukui, Luciane V. Mello, James E Littlejohn, Barbara Setlow, Peter Setlow, Kijeong Kim, Terrance Leighton, Mark J. Jedrzejas

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phosphoglycerate mutases (PGMs) catalyze the isomerization of 2- and 3-phosphoglycerates and are essential for glucose metabolism in most organisms. This study reports the production, structure, and molecular dynamics analysis of Bacillus anthracis cofactor-independent PGM (iPGM). The three-dimensional structure of B. anthracis PGM is composed of two structural and functional domains, the phosphatase and transferase. The structural relationship between these two domains is different than in the B. stearothermophilus iPGM structure determined previously. However, the structures of the two domains of B. anthracis iPGM show a high degree of similarity to those in B. stearothermophilus iPGM. The novel domain arrangement in B. anthracis iPGM and the dynamic property of these domains is directly linked to the mechanism of enzyme catalysis, in which substrate binding is proposed to result in close association of the two domains. The structure of B. anthracis iPGM and the molecular dynamics of this structure provide unique insight into the mechanism of iPGM catalysis, in particular the roles of changes in coordination geometry of the enzyme's two bivalent metal ions and the regulation of this enzyme's activity by changes in intracellular pH during spore formation and germination in Bacillus species.

Original languageEnglish (US)
Pages (from-to)977-988
Number of pages12
JournalBiophysical Journal
Volume92
Issue number3
DOIs
StatePublished - Feb 2007
Externally publishedYes

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Phosphoglycerate Mutase
Bacillus anthracis
Molecular Structure
Spores
Bacillus
Enzymes
Molecular Dynamics Simulation
Catalysis
Germination
Transferases
Phosphoric Monoester Hydrolases
Metals
Ions
Glucose

ASJC Scopus subject areas

  • Biophysics

Cite this

Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase : A crucial enzyme for spores and growing cells of Bacillus species. / Nukui, Masatoshi; Mello, Luciane V.; Littlejohn, James E; Setlow, Barbara; Setlow, Peter; Kim, Kijeong; Leighton, Terrance; Jedrzejas, Mark J.

In: Biophysical Journal, Vol. 92, No. 3, 02.2007, p. 977-988.

Research output: Contribution to journalArticle

Nukui, Masatoshi ; Mello, Luciane V. ; Littlejohn, James E ; Setlow, Barbara ; Setlow, Peter ; Kim, Kijeong ; Leighton, Terrance ; Jedrzejas, Mark J. / Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase : A crucial enzyme for spores and growing cells of Bacillus species. In: Biophysical Journal. 2007 ; Vol. 92, No. 3. pp. 977-988.
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