Abstract
The M2 double-stranded (da) RNA apeciea encodes toxin and resistance functions in Saccharomyces cereviaiae straina with the K2 killer specificity. RNA sequence analysis reveals the presence of a large open reading frame on the larger heat-cleavage product of M2 dsRNA, which is translated in vitro to yield a 28 kd polypeptide as a major product. The postulated translation initiator AUG triplet is located within a stem and loop structure near the 5′terminus of the positive strand, which also contains plausible 16S and 5.8S ribosomal RNA binding sites. These features nay serve to regulate the translation of the K2 toxin precursor. The M1 (from type 1 yeast killers) and M2 dsRNA species lack extensive sequence homology, although specific features are shared, which may represent structural elements required for gene expression and replication
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4379-4400 |
| Number of pages | 22 |
| Journal | Nucleic Acids Research |
| Volume | 13 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jun 25 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
- Genetics
Cite this
Structure and expression of the M2 genomic segment of a type 2 killer virus of yeast. / Hannig, Ernest M.; Leibowitz, Michael J.
In: Nucleic Acids Research, Vol. 13, No. 12, 25.06.1985, p. 4379-4400.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structure and expression of the M2 genomic segment of a type 2 killer virus of yeast
AU - Hannig, Ernest M.
AU - Leibowitz, Michael J
PY - 1985/6/25
Y1 - 1985/6/25
N2 - The M2 double-stranded (da) RNA apeciea encodes toxin and resistance functions in Saccharomyces cereviaiae straina with the K2 killer specificity. RNA sequence analysis reveals the presence of a large open reading frame on the larger heat-cleavage product of M2 dsRNA, which is translated in vitro to yield a 28 kd polypeptide as a major product. The postulated translation initiator AUG triplet is located within a stem and loop structure near the 5′terminus of the positive strand, which also contains plausible 16S and 5.8S ribosomal RNA binding sites. These features nay serve to regulate the translation of the K2 toxin precursor. The M1 (from type 1 yeast killers) and M2 dsRNA species lack extensive sequence homology, although specific features are shared, which may represent structural elements required for gene expression and replication
AB - The M2 double-stranded (da) RNA apeciea encodes toxin and resistance functions in Saccharomyces cereviaiae straina with the K2 killer specificity. RNA sequence analysis reveals the presence of a large open reading frame on the larger heat-cleavage product of M2 dsRNA, which is translated in vitro to yield a 28 kd polypeptide as a major product. The postulated translation initiator AUG triplet is located within a stem and loop structure near the 5′terminus of the positive strand, which also contains plausible 16S and 5.8S ribosomal RNA binding sites. These features nay serve to regulate the translation of the K2 toxin precursor. The M1 (from type 1 yeast killers) and M2 dsRNA species lack extensive sequence homology, although specific features are shared, which may represent structural elements required for gene expression and replication
UR - http://www.scopus.com/inward/record.url?scp=0022432239&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022432239&partnerID=8YFLogxK
U2 - 10.1093/nar/13.12.4379
DO - 10.1093/nar/13.12.4379
M3 - Article
C2 - 3892487
AN - SCOPUS:0022432239
VL - 13
SP - 4379
EP - 4400
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 12
ER -