Structure and expression of the M2 genomic segment of a type 2 killer virus of yeast

Ernest M. Hannig, Michael J Leibowitz

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The M2 double-stranded (da) RNA apeciea encodes toxin and resistance functions in Saccharomyces cereviaiae straina with the K2 killer specificity. RNA sequence analysis reveals the presence of a large open reading frame on the larger heat-cleavage product of M2 dsRNA, which is translated in vitro to yield a 28 kd polypeptide as a major product. The postulated translation initiator AUG triplet is located within a stem and loop structure near the 5′terminus of the positive strand, which also contains plausible 16S and 5.8S ribosomal RNA binding sites. These features nay serve to regulate the translation of the K2 toxin precursor. The M1 (from type 1 yeast killers) and M2 dsRNA species lack extensive sequence homology, although specific features are shared, which may represent structural elements required for gene expression and replication

Original languageEnglish (US)
Pages (from-to)4379-4400
Number of pages22
JournalNucleic Acids Research
Volume13
Issue number12
DOIs
StatePublished - Jun 25 1985
Externally publishedYes

Fingerprint

5.8S Ribosomal RNA
16S Ribosomal RNA
RNA Sequence Analysis
Saccharomyces
Double-Stranded RNA
Sequence Homology
RNA
Viruses
Yeast
Open Reading Frames
Virus
Genomics
Hot Temperature
Yeasts
Binding Sites
Gene Expression
Peptides
Polypeptides
Sequence Analysis
Binding sites

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Structure and expression of the M2 genomic segment of a type 2 killer virus of yeast. / Hannig, Ernest M.; Leibowitz, Michael J.

In: Nucleic Acids Research, Vol. 13, No. 12, 25.06.1985, p. 4379-4400.

Research output: Contribution to journalArticle

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