Structure and expression of proteolipid protein in the peripheral nervous system

J. Kamholz, M. Sessa, S. Scherer, H. Vogelbacker, K. Mokuno, P. Baron, L. Wrabetz, M. Shy, David E Pleasure

Research output: Contribution to journalArticle

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Abstract

Proteolipid protein (PLP), the major myelin protein in the central nervous system (CNS), is also made by Schwann cells (SC) in the peripheral nervous system (PNS) but is not incorporated into the SC myelin sheath. We analyzed several PLP cDNA clones isolated from a rat sciatic nerve cDNA library and found that their coding sequences were identical to PLP cDNAs previously isolated from the CNS. In addition, we have discovered an unusual form of PLP message, present in both brain and sciatic nerve RNA, that is likely formed by alternative splicing within the 3' untranslated region of the primary PLP transcript. The absence of PLP from the SC myelin sheath thus cannot be explained by an alteration in its amino acid sequence. Steady-state levels of PLP mRNA in SC cultures treated with the cAMP analogue dibutyryl cAMP (dBcAMP) were not increased, whereas dBcAMP increased steady-state levels of mRNA encoding the major myelin protein, P0. We have also shown that expression of PLP, unlike that of P0, is regulated in SC in vitro at a posttranscriptional level. Finally, the steady-state levels of P0 mRNA are much more dramatically reduced than those of PLP mRNA during Wallerian degeneration of the peripheral nerve. Thus PLP expression in the PNS is probably controlled by different molecular mechanisms from P0, and may not be part of the coordinate program of myelin gene expression. In contrast to its expression in the PNS, transcription of PLP in the CNS is coordinately regulated along with the other myelin protein genes, suggesting there may be differences in the cis-acting elements and transacting factors involved in the regulation of PLP transcription in SC and oligodendrocytes (OC). Consistent with this notion, we have found that most PLP transcripts are initiated at the more proximal of two start sites in the PNS, while in the CNS proportionally more PLP transcripts are initiated from the distal start site. We propose that the proximal site, utilized predominantly in SC, is responsible for maintenance expression of PLP and is not inducible, while the distal site is responsible for the rapid, inducible increase of PLP message during brain development.

Original languageEnglish (US)
Pages (from-to)231-244
Number of pages14
JournalJournal of Neuroscience Research
Volume31
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Proteolipids
Peripheral Nervous System
Schwann Cells
Proteins
Central Nervous System
Myelin Sheath
Myelin P0 Protein
Myelin Proteins
Messenger RNA
Sciatic Nerve
Complementary DNA
Wallerian Degeneration
Oligodendroglia
Brain
Alternative Splicing
3' Untranslated Regions

Keywords

  • PLP
  • PNS
  • Schwann cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Structure and expression of proteolipid protein in the peripheral nervous system. / Kamholz, J.; Sessa, M.; Scherer, S.; Vogelbacker, H.; Mokuno, K.; Baron, P.; Wrabetz, L.; Shy, M.; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 31, No. 2, 1992, p. 231-244.

Research output: Contribution to journalArticle

Kamholz, J, Sessa, M, Scherer, S, Vogelbacker, H, Mokuno, K, Baron, P, Wrabetz, L, Shy, M & Pleasure, DE 1992, 'Structure and expression of proteolipid protein in the peripheral nervous system', Journal of Neuroscience Research, vol. 31, no. 2, pp. 231-244. https://doi.org/10.1002/jnr.490310204
Kamholz, J. ; Sessa, M. ; Scherer, S. ; Vogelbacker, H. ; Mokuno, K. ; Baron, P. ; Wrabetz, L. ; Shy, M. ; Pleasure, David E. / Structure and expression of proteolipid protein in the peripheral nervous system. In: Journal of Neuroscience Research. 1992 ; Vol. 31, No. 2. pp. 231-244.
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AU - Sessa, M.

AU - Scherer, S.

AU - Vogelbacker, H.

AU - Mokuno, K.

AU - Baron, P.

AU - Wrabetz, L.

AU - Shy, M.

AU - Pleasure, David E

PY - 1992

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N2 - Proteolipid protein (PLP), the major myelin protein in the central nervous system (CNS), is also made by Schwann cells (SC) in the peripheral nervous system (PNS) but is not incorporated into the SC myelin sheath. We analyzed several PLP cDNA clones isolated from a rat sciatic nerve cDNA library and found that their coding sequences were identical to PLP cDNAs previously isolated from the CNS. In addition, we have discovered an unusual form of PLP message, present in both brain and sciatic nerve RNA, that is likely formed by alternative splicing within the 3' untranslated region of the primary PLP transcript. The absence of PLP from the SC myelin sheath thus cannot be explained by an alteration in its amino acid sequence. Steady-state levels of PLP mRNA in SC cultures treated with the cAMP analogue dibutyryl cAMP (dBcAMP) were not increased, whereas dBcAMP increased steady-state levels of mRNA encoding the major myelin protein, P0. We have also shown that expression of PLP, unlike that of P0, is regulated in SC in vitro at a posttranscriptional level. Finally, the steady-state levels of P0 mRNA are much more dramatically reduced than those of PLP mRNA during Wallerian degeneration of the peripheral nerve. Thus PLP expression in the PNS is probably controlled by different molecular mechanisms from P0, and may not be part of the coordinate program of myelin gene expression. In contrast to its expression in the PNS, transcription of PLP in the CNS is coordinately regulated along with the other myelin protein genes, suggesting there may be differences in the cis-acting elements and transacting factors involved in the regulation of PLP transcription in SC and oligodendrocytes (OC). Consistent with this notion, we have found that most PLP transcripts are initiated at the more proximal of two start sites in the PNS, while in the CNS proportionally more PLP transcripts are initiated from the distal start site. We propose that the proximal site, utilized predominantly in SC, is responsible for maintenance expression of PLP and is not inducible, while the distal site is responsible for the rapid, inducible increase of PLP message during brain development.

AB - Proteolipid protein (PLP), the major myelin protein in the central nervous system (CNS), is also made by Schwann cells (SC) in the peripheral nervous system (PNS) but is not incorporated into the SC myelin sheath. We analyzed several PLP cDNA clones isolated from a rat sciatic nerve cDNA library and found that their coding sequences were identical to PLP cDNAs previously isolated from the CNS. In addition, we have discovered an unusual form of PLP message, present in both brain and sciatic nerve RNA, that is likely formed by alternative splicing within the 3' untranslated region of the primary PLP transcript. The absence of PLP from the SC myelin sheath thus cannot be explained by an alteration in its amino acid sequence. Steady-state levels of PLP mRNA in SC cultures treated with the cAMP analogue dibutyryl cAMP (dBcAMP) were not increased, whereas dBcAMP increased steady-state levels of mRNA encoding the major myelin protein, P0. We have also shown that expression of PLP, unlike that of P0, is regulated in SC in vitro at a posttranscriptional level. Finally, the steady-state levels of P0 mRNA are much more dramatically reduced than those of PLP mRNA during Wallerian degeneration of the peripheral nerve. Thus PLP expression in the PNS is probably controlled by different molecular mechanisms from P0, and may not be part of the coordinate program of myelin gene expression. In contrast to its expression in the PNS, transcription of PLP in the CNS is coordinately regulated along with the other myelin protein genes, suggesting there may be differences in the cis-acting elements and transacting factors involved in the regulation of PLP transcription in SC and oligodendrocytes (OC). Consistent with this notion, we have found that most PLP transcripts are initiated at the more proximal of two start sites in the PNS, while in the CNS proportionally more PLP transcripts are initiated from the distal start site. We propose that the proximal site, utilized predominantly in SC, is responsible for maintenance expression of PLP and is not inducible, while the distal site is responsible for the rapid, inducible increase of PLP message during brain development.

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