Structural organization of the cytoskeleton in SV40 human corneal epithelial cells cultured on nano- and microscale grooves

Nancy W. Karuri, Paul F. Nealey, Christopher J Murphy, Ralph M. Albrecht

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The basement membrane of human corneal epithelial cells (HCECs) has a three-dimensional nanoscale architecture, which includes pores, bumps and fibers that may influence cell-substrate adhesion and spreading in the overlying cells. We previously demonstrated that nano- and microscale groove and ridge patterns influence the morphological response and the adhesive response of HCECs to a nominal wall shear stress. Cell-substrate adhesion is mediated by adhesion receptors that bind to extracellular matrix components and anchor the cytoskeleton (CSK) of cells to extracellular elements. Here we investigate the CSK organization in SV40-transformed HCECs grown on nano- and microscale groove and ridge patterns. X-ray lithography was used to fabricate uniform groove and ridge patterns with features ranging in size from 200 nm to 2 mm grooves. Scanning electron microscopy and transmission electron microscopy were used to investigate CSK structure and the distribution of -β1 integrin adhesion receptors. CSK elements aligned with the patterns; however, the spatial organization of these elements was influenced by feature size. Larger CSK bundles lay on top of the ridges and ran parallel to the patterns, whereas smaller CSK bundles, whose width was proportional to the groove size, spanned the grooves. -β1 integrins co-localized with the CSK and had a higher density at the poles of aligned spindle-shaped cells. Differences in organization seen on the different topographical feature sizes may be indicative of differences in extracellular matrix organization. This may explain, in part, previous observations regarding the dependence of cell adhesive responses on the size of topographic features in the substrate.

Original languageEnglish (US)
Pages (from-to)405-413
Number of pages9
JournalScanning
Volume30
Issue number5
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

cultured cells
grooves
microbalances
Adhesion
ridges
adhesion
cells
Adhesives
Substrates
X ray lithography
adhesives
bundles
Anchors
Shear stress
Poles
spindles
matrices
Transmission electron microscopy
basements
Scanning electron microscopy

Keywords

  • Adhesion
  • Cornea
  • Cytoskeleton
  • Epithelium
  • Filopodia
  • Holes
  • Morphology
  • Nanoscale
  • Pattern
  • Proliferation
  • Topography

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Structural organization of the cytoskeleton in SV40 human corneal epithelial cells cultured on nano- and microscale grooves. / Karuri, Nancy W.; Nealey, Paul F.; Murphy, Christopher J; Albrecht, Ralph M.

In: Scanning, Vol. 30, No. 5, 09.2008, p. 405-413.

Research output: Contribution to journalArticle

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