Structural organization and differential expression of rice α-amylase genes

Ning Huang, Nozomu Koizumi, Stephen Reinl, Raymond L. Rodriguez

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Rice α-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene, RAmy3D, which is identical to a previously characterized cDNA, pOS137. RAmy3D is physically linked to the sole representative of Group 5, RAmy3E. The identity between these two genes is 81.4% in the coding region but less than 50% in the 5′ and 3′ flanking regions. Northern blot analysis and RNA-PCR were used to detect the expression of α-amylase genes in various tissues. Alpha-amylase mRNA was abundant in germinating seeds and callus. Some genes were also expressed at much lower levels in roots, young leaves and immature seeds. RAmy1A and RAmy3E were expressed in all tissues while RAmy3D was expressed in all tissues except the immature seeds. RAmy1B was weakly expressed only in callus. RAmy1A transcript was most abundant in the germinating seeds, while RAmy3D and RAmy3E transcripts were most abundant in callus and immature seeds, respectively.

Original languageEnglish (US)
Pages (from-to)7007-7014
Number of pages8
JournalNucleic Acids Research
Volume18
Issue number23
StatePublished - Dec 11 1990

Fingerprint

Amylases
Differential Expression
Seed
Seeds
Bony Callus
Genes
Callus
Gene
DNA sequences
Tissue
DNA Sequence
3' Flanking Region
Cotyledon
alpha-Amylases
5' Flanking Region
Multigene Family
Southern Blotting
Northern Blotting
CDNA
RNA

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Huang, N., Koizumi, N., Reinl, S., & Rodriguez, R. L. (1990). Structural organization and differential expression of rice α-amylase genes. Nucleic Acids Research, 18(23), 7007-7014.

Structural organization and differential expression of rice α-amylase genes. / Huang, Ning; Koizumi, Nozomu; Reinl, Stephen; Rodriguez, Raymond L.

In: Nucleic Acids Research, Vol. 18, No. 23, 11.12.1990, p. 7007-7014.

Research output: Contribution to journalArticle

Huang, N, Koizumi, N, Reinl, S & Rodriguez, RL 1990, 'Structural organization and differential expression of rice α-amylase genes', Nucleic Acids Research, vol. 18, no. 23, pp. 7007-7014.
Huang N, Koizumi N, Reinl S, Rodriguez RL. Structural organization and differential expression of rice α-amylase genes. Nucleic Acids Research. 1990 Dec 11;18(23):7007-7014.
Huang, Ning ; Koizumi, Nozomu ; Reinl, Stephen ; Rodriguez, Raymond L. / Structural organization and differential expression of rice α-amylase genes. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 23. pp. 7007-7014.
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AB - Rice α-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene, RAmy3D, which is identical to a previously characterized cDNA, pOS137. RAmy3D is physically linked to the sole representative of Group 5, RAmy3E. The identity between these two genes is 81.4% in the coding region but less than 50% in the 5′ and 3′ flanking regions. Northern blot analysis and RNA-PCR were used to detect the expression of α-amylase genes in various tissues. Alpha-amylase mRNA was abundant in germinating seeds and callus. Some genes were also expressed at much lower levels in roots, young leaves and immature seeds. RAmy1A and RAmy3E were expressed in all tissues while RAmy3D was expressed in all tissues except the immature seeds. RAmy1B was weakly expressed only in callus. RAmy1A transcript was most abundant in the germinating seeds, while RAmy3D and RAmy3E transcripts were most abundant in callus and immature seeds, respectively.

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