Structural organization and differential expression of rice α-amylase genes

Ning Huang, Nozomu Koizumi, Stephen Reinl, Raymond L. Rodriguez

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Rice α-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene, RAmy3D, which is identical to a previously characterized cDNA, pOS137. RAmy3D is physically linked to the sole representative of Group 5, RAmy3E. The identity between these two genes is 81.4% in the coding region but less than 50% in the 5′ and 3′ flanking regions. Northern blot analysis and RNA-PCR were used to detect the expression of α-amylase genes in various tissues. Alpha-amylase mRNA was abundant in germinating seeds and callus. Some genes were also expressed at much lower levels in roots, young leaves and immature seeds. RAmy1A and RAmy3E were expressed in all tissues while RAmy3D was expressed in all tissues except the immature seeds. RAmy1B was weakly expressed only in callus. RAmy1A transcript was most abundant in the germinating seeds, while RAmy3D and RAmy3E transcripts were most abundant in callus and immature seeds, respectively.

Original languageEnglish (US)
Pages (from-to)7007-7014
Number of pages8
JournalNucleic Acids Research
Volume18
Issue number23
StatePublished - Dec 11 1990

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Structural organization and differential expression of rice α-amylase genes'. Together they form a unique fingerprint.

  • Cite this

    Huang, N., Koizumi, N., Reinl, S., & Rodriguez, R. L. (1990). Structural organization and differential expression of rice α-amylase genes. Nucleic Acids Research, 18(23), 7007-7014.