Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1

Eva B. Nygaard, Jens O. Lagerstedt, Gabriel Bjerre, Biao Shi, Madhu Budamagunta, Kristian A. Poulsen, Stine Meinild, Robert R. Rigor, John C Voss, Peter M Cala, Stine F. Pedersen

Research output: Contribution to journalArticle

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Abstract

We previously presented evidence that transmembrane domain (TM) IV and TM X-XI are important for inhibitor binding and ion transport by the human Na +/H+ exchanger, hNHE1 (Pedersen, S. F., King, S. A., Nygaard, E. B., Rigor, R. R., and Cala, P. M. (2007) J. Biol. Chem. 282, 19716-19727). Here, we present a structural model of the transmembrane part of hNHE1 that further supports this conclusion. The hNHE1 model was based on the crystal structure of the Escherichia coli Na+/H+ antiporter, NhaA, and previous cysteine scanning accessibility studies of hNHE1 and was validated by EPR spectroscopy of spin labels in TM IV and TM XI, as well as by functional analysis of hNHE1 mutants. Removal of all endogenous cysteines in hNHE1, introduction of the mutations A173C (TM IV) and/or I461C (TM XI), and expression of the constructs in mammalian cells resulted in functional hNHE1 proteins. The distance between these spin labels was ∼15 A, confirming that TM IV and TM XI are in close proximity. This distance was decreased both at pH 5.1 and in the presence of the NHE1 inhibitor cariporide. A similar TM IV·TM XI distance and a similar change upon a pH shift were found for the cariporide-insensitive Pleuronectes americanus (pa) NHE1; however, in paNHE1, cariporide had no effect on TM IV·TM XI distance. The central role of the TM IV·TM XI arrangement was confirmed by the partial loss of function upon mutation of Arg425, which the model predicts stabilizes this arrangement. The data are consistent with a role for TM IV and TM XI rearrangements coincident with ion translocation and inhibitor binding by hNHE1.

Original languageEnglish (US)
Pages (from-to)634-648
Number of pages15
JournalJournal of Biological Chemistry
Volume286
Issue number1
DOIs
StatePublished - Jan 7 2011

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Sodium-Hydrogen Antiporter
Electron Spin Resonance Spectroscopy
Paramagnetic resonance
Spin Labels
Spectrum Analysis
Protein Isoforms
Spectroscopy
Cysteine
Ions
Flounder
Functional analysis
Mutation
Structural Models
Ion Transport
Escherichia coli
Crystal structure
Cells
Scanning
cariporide
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Nygaard, E. B., Lagerstedt, J. O., Bjerre, G., Shi, B., Budamagunta, M., Poulsen, K. A., ... Pedersen, S. F. (2011). Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1. Journal of Biological Chemistry, 286(1), 634-648. https://doi.org/10.1074/jbc.M110.159202

Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1. / Nygaard, Eva B.; Lagerstedt, Jens O.; Bjerre, Gabriel; Shi, Biao; Budamagunta, Madhu; Poulsen, Kristian A.; Meinild, Stine; Rigor, Robert R.; Voss, John C; Cala, Peter M; Pedersen, Stine F.

In: Journal of Biological Chemistry, Vol. 286, No. 1, 07.01.2011, p. 634-648.

Research output: Contribution to journalArticle

Nygaard, EB, Lagerstedt, JO, Bjerre, G, Shi, B, Budamagunta, M, Poulsen, KA, Meinild, S, Rigor, RR, Voss, JC, Cala, PM & Pedersen, SF 2011, 'Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1', Journal of Biological Chemistry, vol. 286, no. 1, pp. 634-648. https://doi.org/10.1074/jbc.M110.159202
Nygaard, Eva B. ; Lagerstedt, Jens O. ; Bjerre, Gabriel ; Shi, Biao ; Budamagunta, Madhu ; Poulsen, Kristian A. ; Meinild, Stine ; Rigor, Robert R. ; Voss, John C ; Cala, Peter M ; Pedersen, Stine F. / Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 1. pp. 634-648.
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AU - Budamagunta, Madhu

AU - Poulsen, Kristian A.

AU - Meinild, Stine

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