Structural evolution of Reoviridae revealed by Oryzavirus in acquiring the second capsid shell

Naoyuki Miyazaki, Tamaki Uehara-Ichiki, Li Xing, Leif Bergman, Akifumi Higashiura, Atsushi Nakagawa, Toshihiro Omura, R. Holland Cheng

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The conservation of the core structure and diversification of the external features among the turreted reoviruses appear to be relevant to structural evolution in facilitating the infection of diverse host species. The structure of Rice ragged stunt virus (RRSV), in the genus Oryzavirus of the family Reoviridae, is determined to show a core composed of capsid shell, clamps, and long turrets. The RRSV core structure is equivalent to the core structure of Orthoreovirus and the virion structure of Cytoplasmic polyhedrosis virus (CPV). In RRSV, five peripheral trimers surround each long turret and sit at the Q trimer position in the T=13l icosahedral symmetry, a structural feature unique to turreted reoviruses. That is, the core of RRSV is partially covered by 60 copies of the peripheral trimer. In contrast, the core of Orthoreovirus is covered by 200 copies of the trimer that sit at the Q, R, S, and T trimer positions. Our results suggest that among the three viruses, RRSV has a structure intermediate between that of Orthoreovirus and the CPV virion. This conclusion coincides with the results of the phylogenetic analysis of amino acid sequences of RNA-dependent RNA polymerases.

Original languageEnglish (US)
Pages (from-to)11344-11353
Number of pages10
JournalJournal of Virology
Volume82
Issue number22
DOIs
StatePublished - Nov 2008

Fingerprint

Oryzavirus
Rice ragged stunt virus
Reoviridae
capsid
Capsid
Orthoreovirus
Viruses
Cypovirus
virion
Virion
RNA-directed RNA polymerase
viral morphology
RNA Replicase
Viral Structures
Protein Sequence Analysis
amino acid sequences
Oryza
viruses
phylogeny
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Miyazaki, N., Uehara-Ichiki, T., Xing, L., Bergman, L., Higashiura, A., Nakagawa, A., ... Cheng, R. H. (2008). Structural evolution of Reoviridae revealed by Oryzavirus in acquiring the second capsid shell. Journal of Virology, 82(22), 11344-11353. https://doi.org/10.1128/JVI.02375-07

Structural evolution of Reoviridae revealed by Oryzavirus in acquiring the second capsid shell. / Miyazaki, Naoyuki; Uehara-Ichiki, Tamaki; Xing, Li; Bergman, Leif; Higashiura, Akifumi; Nakagawa, Atsushi; Omura, Toshihiro; Cheng, R. Holland.

In: Journal of Virology, Vol. 82, No. 22, 11.2008, p. 11344-11353.

Research output: Contribution to journalArticle

Miyazaki, N, Uehara-Ichiki, T, Xing, L, Bergman, L, Higashiura, A, Nakagawa, A, Omura, T & Cheng, RH 2008, 'Structural evolution of Reoviridae revealed by Oryzavirus in acquiring the second capsid shell', Journal of Virology, vol. 82, no. 22, pp. 11344-11353. https://doi.org/10.1128/JVI.02375-07
Miyazaki N, Uehara-Ichiki T, Xing L, Bergman L, Higashiura A, Nakagawa A et al. Structural evolution of Reoviridae revealed by Oryzavirus in acquiring the second capsid shell. Journal of Virology. 2008 Nov;82(22):11344-11353. https://doi.org/10.1128/JVI.02375-07
Miyazaki, Naoyuki ; Uehara-Ichiki, Tamaki ; Xing, Li ; Bergman, Leif ; Higashiura, Akifumi ; Nakagawa, Atsushi ; Omura, Toshihiro ; Cheng, R. Holland. / Structural evolution of Reoviridae revealed by Oryzavirus in acquiring the second capsid shell. In: Journal of Virology. 2008 ; Vol. 82, No. 22. pp. 11344-11353.
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