Structural evidence of glycoprotein assembly in cellular membrane compartments prior to alphavirus budding

Pan Soonsawad, Li Xing, Emerson Milla, Juan M. Espinoza, Masaaki Kawano, Michael Marko, Chyongere Hsieh, Hiromitsu Furukawa, Masahiro Kawasaki, Wattana Weerachatyanukul, Ranjana Srivastava, Susan W. Barnett, Indresh K. Srivastava, R. Holland Cheng

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Membrane glycoproteins of alphavirus play a critical role in the assembly and budding of progeny virions. However, knowledge regarding transport of viral glycoproteins to the plasma membrane is obscure. In this study, we investigated the role of cytopathic vacuole type II (CPV-II) through in situ electron tomography of alphavirus-infected cells. The results revealed that CPV-II contains viral glycoproteins arranged in helical tubular arrays resembling the basic organization of glycoprotein trimers on the envelope of the mature virions. The location of CPV-II adjacent to the site of viral budding suggests a model for the transport of structural components to the site of budding. Thus, the structural characteristics of CPV-II can be used in evaluating the design of a packaging cell line for replicon production.

Original languageEnglish (US)
Pages (from-to)11145-11151
Number of pages7
JournalJournal of Virology
Volume84
Issue number21
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Medicine(all)

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