Abstract
Structural Evaluation of the Respiratory System. TYLER, W. S., DUNGWORTH, D. L., PLOPPER, C. G., HYDE, D. M., AND TYLER, N. K. (1985). Fundam. Appl. Toxicol. 5, 405-422. The theoretical and practical bases for morphological evaluation of the respiratory system useful for inhalation toxicology are reviewed. For most studies we recommend a comprehensive gross examination followed by in vitro tracheal infusion of a fixative containing both glutaral-dehyde and formaldehyde in cacodylate buffer. Lungs fixed in this manner are suitable for LM, SEM, and TEM and lung volumes can be determined. The airway orientation of many lesions and the potential for gradients of damage are considered in the lung sampling plan. While LM of paraffin sections continues to be the basic method for evaluation, the SEM and TEM, especially when ancillary methods are used, provide valuable additional information. The use of backscattered electrons and energy-dispersive X-ray analysis in the SEM provides information concerning the localization and elemental analyses of particles. Cytochemical procedures characterize biological activities of specific cell types and are becoming more widely used. Morphometry permits correlation of quantified structure with physiological and biochemical data.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 405-422 |
| Number of pages | 18 |
| Journal | Toxicological Sciences |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1985 |
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ASJC Scopus subject areas
- Molecular Biology
- Embryology
- Cell Biology
- Genetics
- Developmental Biology
- Toxicology
Cite this
Structural evaluation of the respiratory system. / Tyler, Walter S.; Dungworth, Donald L.; Plopper, Charles; Hyde, Dallas M.; Tyler, Nancy K.
In: Toxicological Sciences, Vol. 5, No. 3, 06.1985, p. 405-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structural evaluation of the respiratory system
AU - Tyler, Walter S.
AU - Dungworth, Donald L.
AU - Plopper, Charles
AU - Hyde, Dallas M.
AU - Tyler, Nancy K.
PY - 1985/6
Y1 - 1985/6
N2 - Structural Evaluation of the Respiratory System. TYLER, W. S., DUNGWORTH, D. L., PLOPPER, C. G., HYDE, D. M., AND TYLER, N. K. (1985). Fundam. Appl. Toxicol. 5, 405-422. The theoretical and practical bases for morphological evaluation of the respiratory system useful for inhalation toxicology are reviewed. For most studies we recommend a comprehensive gross examination followed by in vitro tracheal infusion of a fixative containing both glutaral-dehyde and formaldehyde in cacodylate buffer. Lungs fixed in this manner are suitable for LM, SEM, and TEM and lung volumes can be determined. The airway orientation of many lesions and the potential for gradients of damage are considered in the lung sampling plan. While LM of paraffin sections continues to be the basic method for evaluation, the SEM and TEM, especially when ancillary methods are used, provide valuable additional information. The use of backscattered electrons and energy-dispersive X-ray analysis in the SEM provides information concerning the localization and elemental analyses of particles. Cytochemical procedures characterize biological activities of specific cell types and are becoming more widely used. Morphometry permits correlation of quantified structure with physiological and biochemical data.
AB - Structural Evaluation of the Respiratory System. TYLER, W. S., DUNGWORTH, D. L., PLOPPER, C. G., HYDE, D. M., AND TYLER, N. K. (1985). Fundam. Appl. Toxicol. 5, 405-422. The theoretical and practical bases for morphological evaluation of the respiratory system useful for inhalation toxicology are reviewed. For most studies we recommend a comprehensive gross examination followed by in vitro tracheal infusion of a fixative containing both glutaral-dehyde and formaldehyde in cacodylate buffer. Lungs fixed in this manner are suitable for LM, SEM, and TEM and lung volumes can be determined. The airway orientation of many lesions and the potential for gradients of damage are considered in the lung sampling plan. While LM of paraffin sections continues to be the basic method for evaluation, the SEM and TEM, especially when ancillary methods are used, provide valuable additional information. The use of backscattered electrons and energy-dispersive X-ray analysis in the SEM provides information concerning the localization and elemental analyses of particles. Cytochemical procedures characterize biological activities of specific cell types and are becoming more widely used. Morphometry permits correlation of quantified structure with physiological and biochemical data.
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UR - http://www.scopus.com/inward/citedby.url?scp=77957186022&partnerID=8YFLogxK
U2 - 10.1093/toxsci/5.3.405
DO - 10.1093/toxsci/5.3.405
M3 - Article
C2 - 2408953
AN - SCOPUS:77957186022
VL - 5
SP - 405
EP - 422
JO - Toxicological Sciences
JF - Toxicological Sciences
SN - 1096-6080
IS - 3
ER -