Structural domains of the avian erythroblastosis virus erbB protein required for fibroblast transformation: Dissection by in-frame insertional mutagenesis

M. Ng, M. L. Privalsky

Research output: Contribution to journalArticle

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Abstract

Avian erythroblastosis virus (AEV) induces erythroblastosis and fibrosarcomas. The viral erbB protein is required for AEV-mediated oncogenesis. To explore the structural aspects of the v-erbB polypeptide necessary for its oncogenic function, we created a series of small in-frame insertions in different domains of the v-erbB oncogene. AEV genomes bearing lesions within the v-erbB kinase domain demonstrated a drastically decreased ability to transform avian fibroblasts, establishing a functional role for this structurally conserved oncogene domain. In contrast, mutations in the extracellular domain, between the transmembrane region and the kinase domain, or at the extreme C terminus of the v-erbB protein had no effect on AEV-mediated fibroblast transformation. One lesion within the v-erbB kinase domain, a 10-amino acid insertion, produced a temperature-sensitive mutant capable of fibroblast transformation at 36°C but not at 41°C, suggesting that small in-frame insertions have general utility for the in vitro creation of conditional mutants.

Original languageEnglish (US)
Pages (from-to)542-553
Number of pages12
JournalJournal of Virology
Volume58
Issue number2
StatePublished - 1986

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ASJC Scopus subject areas

  • Immunology

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