Structural and functional studies of the measles virus hemagglutinin: Identification of a novel site required for CD46 interaction

John B. Patterson, Friedrich Scheiflinger, Marianne Manchester, Tilahun Yilma, Michael B A Oldstone

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The entry of measles virus (MV) into human cells is mediated by the initial attachment of the viral hemagglutinin (HA) to the complement regulatory protein CD46. Two subdomains, one each within CD46 short consensus repeats (SCRs) 1 and 2, are responsible for this interaction. However, little is known about the regions within MV HA needed for a high-affinity CD46 interaction. To better define the HA-CD46 interaction, we took three approaches: chimeric domain swapping, peptide scanning, and alanine scanning mutagenesis. Chimeras of MV HA and the closely related rinderpest virus (RPV) HA were generated and tested for cell surface expression and the ability to hemadsorb CD46+ red blood cells (RBC). Exchanges with the N terminus of RPV were tolerated as MV HA could be replaced with RPV HA up to amino-acid position 154. However, both larger swaps with RPV and a small RPV HA replacement at the C terminus aborted cell-surface expression. Peptide scanning with 51 overlapping peptides derived from three MV HA regions showed one peptide, corresponding to MV HA amino acids 468-487, blocked hemagglutination of African green monkey (AGM) RBCs and inhibited MV infection of Chinese hamster ovary cells (CHO) expressing human CD46. Alanine scanning mutants mapped sites on the MV HA that were not required for trafficking to the cell surface or function in hemagglutination as well as a novel site required for CD46 interaction, amino acids 473-477.

Original languageEnglish (US)
Pages (from-to)142-151
Number of pages10
JournalVirology
Volume256
Issue number1
DOIs
StatePublished - Mar 30 1999

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Measles virus
Hemagglutinins
Rinderpest virus
Hemagglutination
Amino Acids
Alanine
Peptides
Viral Hemagglutinins
Cercopithecus aethiops
Virus Diseases
Cricetulus
Mutagenesis
Ovary
Complement System Proteins
Erythrocytes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Structural and functional studies of the measles virus hemagglutinin : Identification of a novel site required for CD46 interaction. / Patterson, John B.; Scheiflinger, Friedrich; Manchester, Marianne; Yilma, Tilahun; Oldstone, Michael B A.

In: Virology, Vol. 256, No. 1, 30.03.1999, p. 142-151.

Research output: Contribution to journalArticle

Patterson, John B. ; Scheiflinger, Friedrich ; Manchester, Marianne ; Yilma, Tilahun ; Oldstone, Michael B A. / Structural and functional studies of the measles virus hemagglutinin : Identification of a novel site required for CD46 interaction. In: Virology. 1999 ; Vol. 256, No. 1. pp. 142-151.
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