Structural and functional human retinal imaging with a fiber-based visible light OCT ophthalmoscope

Shau Poh Chong, Marcel Bernucci, Harsha Radhakrishnan, Vivek Srinivasan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The design of a multi-functional fiber-based Optical Coherence Tomography (OCT) system for human retinal imaging with < 2 micron axial resolution in tissue is described. A detailed noise characterization of two supercontinuum light sources with different pulse repetition rates is presented. The higher repetition rate and lower noise source is found to enable a sensitivity of 96 dB with 0.15 mW light power at the cornea and a 98 microsecond exposure time. Using a broadband (560 ± 50 nm), 90/10, fused single-mode fiber coupler designed for visiblewavelengths, the sample arm is integrated into an ophthalmoscope platform, similar to current clinical OCT systems. To demonstrate the instrument±s range of operation, in vivo structural retinal imaging is also shown at 0.15 mW exposure with 10,000 and 70,000 axial scans per second (the latter comparable to commercial OCT systems), and at 0.03 mW exposure and 10,000 axial scans per second (below maximum permissible continuous exposure levels). Lastly, in vivo spectroscopic imaging of anatomy, saturation, and hemoglobin content in the human retina is also demonstrated.

Original languageEnglish (US)
Article number#278483
Pages (from-to)323-337
Number of pages15
JournalBiomedical Optics Express
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Ophthalmoscopes
Optical Coherence Tomography
tomography
Light
fibers
Noise
Maximum Allowable Concentration
Cornea
cornea
Retina
retina
anatomy
pulse repetition rate
Anatomy
hemoglobin
Hemoglobins
Arm
Heart Rate
low noise
couplers

Keywords

  • Fiber optics imaging
  • Medical and biological imaging
  • Optical coherence tomography
  • Spectroscopy, speckle
  • Visible lasers

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Structural and functional human retinal imaging with a fiber-based visible light OCT ophthalmoscope. / Chong, Shau Poh; Bernucci, Marcel; Radhakrishnan, Harsha; Srinivasan, Vivek.

In: Biomedical Optics Express, Vol. 8, No. 1, #278483, 01.01.2017, p. 323-337.

Research output: Contribution to journalArticle

Chong, Shau Poh ; Bernucci, Marcel ; Radhakrishnan, Harsha ; Srinivasan, Vivek. / Structural and functional human retinal imaging with a fiber-based visible light OCT ophthalmoscope. In: Biomedical Optics Express. 2017 ; Vol. 8, No. 1. pp. 323-337.
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