Structural and functional analysis of separated strands of killer double-stranded RNA of yeast

Dennis J. Thiele, Michael J Leibowitz

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The two strands of the M double-stranded RNA species from a killer strain of Saccharomyces cerevisiae have been separated, and the 3′-terminal sequences of these strands have been determined. The positive strand programs the synthesis of the putative killer toxin precursor (M-p32) in a rabbit reticulocyte in vitro translation system. Only the negative strand hybridizes to the positive polarity transcript (m) synthesized in vitro by the virion-associated transcriptase activity. Secondary structural analysis of the extreme 3′-terminus of the negative strand using S1 nuclease is consistent with the presence of a large stem and loop structure previously proposed on the basis of RNA sequence data. This structure, and a similar structure at the corresponding 5′-terminus of the positive strand, may have functional significance in vivo.

Original languageEnglish (US)
Pages (from-to)6903-6918
Number of pages16
JournalNucleic Acids Research
Volume10
Issue number21
DOIs
StatePublished - Nov 11 1982
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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