Structural and biochemical changes in lungs of 3-methylindole-treated rats

Leslie Woods, Dennis W Wilson, Mary J. Schiedt, Shri N. Giri

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Effects of a single dose of 3-methylindole (3-MI) (250 mg/kg intraperitoneally) were studied at different times ranging from 12 hours to 2 weeks post-treatment (PT). Microscopic study revealed mild Clara cell injury 24 hours PT and mucus hyperplasia 24 hours to 2 weeks PT. Diffuse type I alveolar epithelial cell necrosis occurred at 48 hours, followed by type II cell hyperplasia. Septal edema and accumulation of interstitial and capillary polymorphonuclear leukocytes and perivascular mixed mononuclear inflammatory cells accompanied the injury and repair. A gradual resolution of lesions with persistent mononuclear inflammatory cellular clusters at septal junctions, focal septal fibrosis, and accumulation of alveolar macrophages was evident at 1 and 2 weeks PT. Collagen, measured as hydroxyproline, in 3-MI-treated rats was significantly increased to 130% and 139% of control (3.0 mg/lung) at 1 and 2 weeks PT, respectively. Biphasic peaks of plasma 6-keto-prostaglandin F occurred at 12 to 24 hours and at 96 hours PT with 3-MI and thromboxane B2 was elevated 12, 48, and 96 hours PT. Right ventricular/left ventricular and septal weight was increased to 120% and 140% of the control 1 and 2 weeks PT. We concluded that 3-MI induces alveolar septal injury in the rat with relatively complete repair of the alveolar epithelium and residual mild focal septal fibrosis and pulmonary hypertension 2 weeks PT. Arachidonic acid-derived mediators and inflammation are associated with 3-MI-induced lung injury.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalAmerican Journal of Pathology
Volume142
Issue number1
StatePublished - Jan 1993

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Skatole
Lung
Hyperplasia
Wounds and Injuries
Fibrosis
Alveolar Epithelial Cells
Inflammation Mediators
Thromboxane B2
Hydroxyproline
Alveolar Macrophages
Lung Injury
Mucus
Pulmonary Hypertension
Arachidonic Acid
Edema
Neutrophils
Necrosis
Collagen
Epithelium
Weights and Measures

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Structural and biochemical changes in lungs of 3-methylindole-treated rats. / Woods, Leslie; Wilson, Dennis W; Schiedt, Mary J.; Giri, Shri N.

In: American Journal of Pathology, Vol. 142, No. 1, 01.1993, p. 129-138.

Research output: Contribution to journalArticle

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