Structural analysis of sulfated O-linked oligosaccharides in glycoproteins by MALDI-FTMS

Yongming Xie, Jerry L. Hedrick, Carlito B Lebrilla

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The structure of sulfated o-linked oligosaccharides in glycoproteins was studied using MALDI-FTMS. O-linked oligosaccharides were released from jelly glycoproteins of X. laevis eggs by alkaline borohydride reduction. A new high performance liquid chromatography (HPLC) method was developed on a porous graphitized carbon column to separate the mixture into small pools containing one or few components. HPLC effluents were collected and analyzed by MALDI-FTMS in both positive and negative modes. The high mass accuracy of FTMS helped in the identification of four of the six reference compounds since their masses were the only ones observed.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages399-400
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Xie, Y., Hedrick, J. L., & Lebrilla, C. B. (2002). Structural analysis of sulfated O-linked oligosaccharides in glycoproteins by MALDI-FTMS. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 399-400)