Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID

Jennifer T. Aguilan, Fabian M. Dayrit, Jinhua Zhang, Milady R. Niñonuevo, Carlito B Lebrilla

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.

Original languageEnglish (US)
Pages (from-to)96-103
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume17
Issue number1
DOIs
StatePublished - Jan 2006

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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