Stress induction of Bm1 RNA in silkworm larvae: SINEs, an unusual class of stress genes

Richard H. Kimura, Prabhakara V Choudary, Koni K. Stone, Carl W. Schmid

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

This study surveys the induction of RNA polymerase III (Pol III)-directed expression of short interspersed element (SINE) transcripts by various stresses in an animal model, silkworm larvae. Sublethal heat shock and exposure to several toxic compounds increase the level of Bm1 RNA, the silkworm SINE transcript, while also transiently increasing expression of a well-characterized stress-induced transcript, Hsp70 messenger RNA (mRNA). In certain cases, the Bm1 RNA response coincides with that of Hsp70 mRNA, but more often Bm1 RNA responds later in recovery. Baculovirus infection and exposure to certain toxic compounds increase Bm1 RNA but not Hsp70 mRNA, showing that SINE induction is not necessarily coupled to transcription of this particular heat shock gene. SINEs behave as an additional class of stress-inducible genes in living animals but are unusual as stress genes because of their high copy number, genomic dispersion, and Pol III-directed transcription.

Original languageEnglish (US)
Pages (from-to)263-272
Number of pages10
JournalCell Stress and Chaperones
Volume6
Issue number3
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Clinical Biochemistry

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