Abstract
Fumarate hydratase (FH) is an enzyme of the mitochondrial tricarboxylic acid cycle (TCAC). Here we report the characterization of a novel FH variant (FHv) that contains an alternative exon lb, thus lacking the mitochondrial signal sequence. Distinct from mitochondrial FH, FHv localized to cytosol and nucleus and lacked FH enzyme activity. FHv was expressed ubiquitously in human fetal and adult tissues. Heat shock and prolonged hypoxia increased FHv expression in a cell line (HTB115) by nine- and fourfold, respectively. These results suggest that FHv has an alternative function outside the TCAC related to cellular stress response.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 59-69 |
| Number of pages | 11 |
| Journal | Gene Expression |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2007 |
| Externally published | Yes |
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Keywords
- Alternative exon
- Fumarate hydratase (FH)
- Heat shock
- Hereditary leiomyomatosis and renal cell cancer (HLRCC)
- Hypoxia
- Stress
- Tricarboxylic acid cycle (TCAC)
ASJC Scopus subject areas
- Medicine(all)
- Molecular Biology
- Genetics
Cite this
Stress-induced expression of a novel variant of human fumarate hydratase (FH). / Lehtonen, Heli J.; Ylisaukko-Oja, Sanna K.; Kiuru, Maija Ht; Karhu, Auli; Lehtonen, Rainer; Vanharanta, Sakari; Jalanko, A. N.U.; Aaltonen, Lauri A.; Launonen, Virpi.
In: Gene Expression, Vol. 14, No. 2, 2007, p. 59-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stress-induced expression of a novel variant of human fumarate hydratase (FH)
AU - Lehtonen, Heli J.
AU - Ylisaukko-Oja, Sanna K.
AU - Kiuru, Maija Ht
AU - Karhu, Auli
AU - Lehtonen, Rainer
AU - Vanharanta, Sakari
AU - Jalanko, A. N.U.
AU - Aaltonen, Lauri A.
AU - Launonen, Virpi
PY - 2007
Y1 - 2007
N2 - Fumarate hydratase (FH) is an enzyme of the mitochondrial tricarboxylic acid cycle (TCAC). Here we report the characterization of a novel FH variant (FHv) that contains an alternative exon lb, thus lacking the mitochondrial signal sequence. Distinct from mitochondrial FH, FHv localized to cytosol and nucleus and lacked FH enzyme activity. FHv was expressed ubiquitously in human fetal and adult tissues. Heat shock and prolonged hypoxia increased FHv expression in a cell line (HTB115) by nine- and fourfold, respectively. These results suggest that FHv has an alternative function outside the TCAC related to cellular stress response.
AB - Fumarate hydratase (FH) is an enzyme of the mitochondrial tricarboxylic acid cycle (TCAC). Here we report the characterization of a novel FH variant (FHv) that contains an alternative exon lb, thus lacking the mitochondrial signal sequence. Distinct from mitochondrial FH, FHv localized to cytosol and nucleus and lacked FH enzyme activity. FHv was expressed ubiquitously in human fetal and adult tissues. Heat shock and prolonged hypoxia increased FHv expression in a cell line (HTB115) by nine- and fourfold, respectively. These results suggest that FHv has an alternative function outside the TCAC related to cellular stress response.
KW - Alternative exon
KW - Fumarate hydratase (FH)
KW - Heat shock
KW - Hereditary leiomyomatosis and renal cell cancer (HLRCC)
KW - Hypoxia
KW - Stress
KW - Tricarboxylic acid cycle (TCAC)
UR - http://www.scopus.com/inward/record.url?scp=38949206304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38949206304&partnerID=8YFLogxK
U2 - 10.3727/105221607783417592
DO - 10.3727/105221607783417592
M3 - Article
VL - 14
SP - 59
EP - 69
JO - Gene Expression
JF - Gene Expression
SN - 1052-2166
IS - 2
ER -