Stress-induced expression of a novel variant of human fumarate hydratase (FH)

Heli J. Lehtonen, Sanna K. Ylisaukko-Oja, Maija Ht Kiuru, Auli Karhu, Rainer Lehtonen, Sakari Vanharanta, A. N.U. Jalanko, Lauri A. Aaltonen, Virpi Launonen

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Fumarate hydratase (FH) is an enzyme of the mitochondrial tricarboxylic acid cycle (TCAC). Here we report the characterization of a novel FH variant (FHv) that contains an alternative exon lb, thus lacking the mitochondrial signal sequence. Distinct from mitochondrial FH, FHv localized to cytosol and nucleus and lacked FH enzyme activity. FHv was expressed ubiquitously in human fetal and adult tissues. Heat shock and prolonged hypoxia increased FHv expression in a cell line (HTB115) by nine- and fourfold, respectively. These results suggest that FHv has an alternative function outside the TCAC related to cellular stress response.

Original languageEnglish (US)
Pages (from-to)59-69
Number of pages11
JournalGene Expression
Volume14
Issue number2
DOIs
StatePublished - 2007
Externally publishedYes

Keywords

  • Alternative exon
  • Fumarate hydratase (FH)
  • Heat shock
  • Hereditary leiomyomatosis and renal cell cancer (HLRCC)
  • Hypoxia
  • Stress
  • Tricarboxylic acid cycle (TCAC)

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

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  • Cite this

    Lehtonen, H. J., Ylisaukko-Oja, S. K., Kiuru, M. H., Karhu, A., Lehtonen, R., Vanharanta, S., Jalanko, A. N. U., Aaltonen, L. A., & Launonen, V. (2007). Stress-induced expression of a novel variant of human fumarate hydratase (FH). Gene Expression, 14(2), 59-69. https://doi.org/10.3727/105221607783417592