Stress failure of pulmonary capillaries in racehorses with exercise- induced pulmonary hemorrhage

J. B. West, O. Mathieu-Costello, James H Jones, E. K. Birks, R. B. Logemann, John Pascoe, W. S. Tyler

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

Bleeding into the lungs in thoroughbreds is extremely common; there is evidence that it occurs in essentially all horses in training. However, the mechanism is unknown. We tested the hypothesis that exercise-induced pulmonary hemorrhage (EIPH) is caused by stress failure of pulmonary capillaries. Three thoroughbreds with known EIPH were galloped on a treadmill, and after the horses were killed with intravenous barbiturate the lungs were removed, inflated, and fixed for electron microscopy. Ultrastructural studies showed evidence of stress failure of pulmonary capillaries, including disruptions of the capillary endothelial and alveolar epithelial layers, extensive collections of red blood cells in the alveolar wall interstitium, proteinaceous fluid and red blood cells in the alveolar spaces, interstitial edema, and fluid-filled protrusions of the endothelium into the capillary lumen. The appearances were consistent with the ultrastructural changes we have previously described in rabbit lungs at high capillary transmural pressures. Actual breaks in the endothelium and epithelium were rather difficult to find, and they were frequently associated with platelets and leukocytes that appeared to be plugging the breaks. The paucity of breaks was ascribed to their reversibility when the pressure was lowered and to the fact that 60-70 min elapsed between the gallop and the beginning of lung fixation. Capillary wall stress was calculated from pulmonary vascular pressures measured in a companion study (Jones et al. FASEB J. 6: A2020, 1992) and from measurements of the thickness of the blood- gas barrier and the radius of curvature of the capillaries. The value was as high as 8 x 105 dyn/cm2 (8 x 104 N/m2), which exceeds the breaking stress of most soft tissues. We conclude that stress failure of pulmonary capillaries is the mechanism of EIPH.

Original languageEnglish (US)
Pages (from-to)1097-1109
Number of pages13
JournalJournal of Applied Physiology
Volume75
Issue number3
StatePublished - 1993

Fingerprint

Hemorrhage
Lung
Pressure
Horses
Erythrocytes
Extracellular Fluid
Vascular Endothelium
Endothelium
Blood Vessels
Edema
Electron Microscopy
Leukocytes
Blood Platelets
Epithelium
Gases
Rabbits

Keywords

  • capillary pressure
  • capillary wall stress
  • furosemide
  • lung bleeding
  • overinflation of lung
  • pulmonary vascular pressures
  • thoroughbred

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

West, J. B., Mathieu-Costello, O., Jones, J. H., Birks, E. K., Logemann, R. B., Pascoe, J., & Tyler, W. S. (1993). Stress failure of pulmonary capillaries in racehorses with exercise- induced pulmonary hemorrhage. Journal of Applied Physiology, 75(3), 1097-1109.

Stress failure of pulmonary capillaries in racehorses with exercise- induced pulmonary hemorrhage. / West, J. B.; Mathieu-Costello, O.; Jones, James H; Birks, E. K.; Logemann, R. B.; Pascoe, John; Tyler, W. S.

In: Journal of Applied Physiology, Vol. 75, No. 3, 1993, p. 1097-1109.

Research output: Contribution to journalArticle

West, JB, Mathieu-Costello, O, Jones, JH, Birks, EK, Logemann, RB, Pascoe, J & Tyler, WS 1993, 'Stress failure of pulmonary capillaries in racehorses with exercise- induced pulmonary hemorrhage', Journal of Applied Physiology, vol. 75, no. 3, pp. 1097-1109.
West, J. B. ; Mathieu-Costello, O. ; Jones, James H ; Birks, E. K. ; Logemann, R. B. ; Pascoe, John ; Tyler, W. S. / Stress failure of pulmonary capillaries in racehorses with exercise- induced pulmonary hemorrhage. In: Journal of Applied Physiology. 1993 ; Vol. 75, No. 3. pp. 1097-1109.
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