Abstract
Using streptavidin as a molecular target for synthetic peptide library screening, ligands with consensus sequences of HPQ and HPM were rapidly identified. Here, we report the discovery of several non-HPQ sequences that were found when a pentapeptide library that lacked histidine was used in the screen. Although the high-affinity natural ligand for both streptavidin and avidin is biotin, neither HPQ nor HPM sequences were discovered when avidin was used in the screen. Instead, a new motif, HP(Y/F)P, was identified. Data obtained from such peptide library screens will help us to better understand molecular recognition.
Original language | English (US) |
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Pages (from-to) | 11-15 |
Number of pages | 5 |
Journal | ImmunoMethods |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - 1992 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology