Abstract
Using streptavidin as a molecular target for synthetic peptide library screening, ligands with consensus sequences of HPQ and HPM were rapidly identified. Here, we report the discovery of several non-HPQ sequences that were found when a pentapeptide library that lacked histidine was used in the screen. Although the high-affinity natural ligand for both streptavidin and avidin is biotin, neither HPQ nor HPM sequences were discovered when avidin was used in the screen. Instead, a new motif, HP(Y/F)P, was identified. Data obtained from such peptide library screens will help us to better understand molecular recognition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-15 |
| Number of pages | 5 |
| Journal | ImmunoMethods |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Streptavidin and avidin recognize peptide ligands with different motifs. / Lam, Kit; Lebl, Michal.
In: ImmunoMethods, Vol. 1, No. 1, 1992, p. 11-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Streptavidin and avidin recognize peptide ligands with different motifs
AU - Lam, Kit
AU - Lebl, Michal
PY - 1992
Y1 - 1992
N2 - Using streptavidin as a molecular target for synthetic peptide library screening, ligands with consensus sequences of HPQ and HPM were rapidly identified. Here, we report the discovery of several non-HPQ sequences that were found when a pentapeptide library that lacked histidine was used in the screen. Although the high-affinity natural ligand for both streptavidin and avidin is biotin, neither HPQ nor HPM sequences were discovered when avidin was used in the screen. Instead, a new motif, HP(Y/F)P, was identified. Data obtained from such peptide library screens will help us to better understand molecular recognition.
AB - Using streptavidin as a molecular target for synthetic peptide library screening, ligands with consensus sequences of HPQ and HPM were rapidly identified. Here, we report the discovery of several non-HPQ sequences that were found when a pentapeptide library that lacked histidine was used in the screen. Although the high-affinity natural ligand for both streptavidin and avidin is biotin, neither HPQ nor HPM sequences were discovered when avidin was used in the screen. Instead, a new motif, HP(Y/F)P, was identified. Data obtained from such peptide library screens will help us to better understand molecular recognition.
UR - http://www.scopus.com/inward/record.url?scp=0002484110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0002484110&partnerID=8YFLogxK
U2 - 10.1016/S1058-6687(05)80023-3
DO - 10.1016/S1058-6687(05)80023-3
M3 - Article
AN - SCOPUS:0002484110
VL - 1
SP - 11
EP - 15
JO - Methods
JF - Methods
SN - 0076-6879
IS - 1
ER -