Strength of multiple parallel biological bonds

Todd Sulchek, Raymond W. Friddle, Aleksandr Noy

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


Multivalent interactions play a critical role in a variety of biological processes on both molecular and cellular levels. We have used molecular force spectroscopy to investigate the strength of multiple parallel peptide-antibody bonds using a system that allowed us to determine the rupture forces and the number of ruptured bonds independently. In our experiments the interacting molecules were attached to the surfaces of the probe and sample of the atomic force microscope with flexible polymer tethers, and the unique mechanical signatures of the tethers determined the number of ruptured bonds. We show that the rupture forces increase with the number of interacting molecules and that the measured forces obey the predictions of a Markovian model for the strength of multiple parallel bonds. We also discuss the implications of our results to the interpretation of force spectroscopy measurements in multiple bond systems.

Original languageEnglish (US)
Pages (from-to)4686-4691
Number of pages6
JournalBiophysical Journal
Issue number12
StatePublished - Jun 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics


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