Abstract
Two new strategies for increasing tumor uptake have been investigated. First the effect of interleukin-2 (IL-2) on tumor uptake of 125I-Lym-l antibody in nude mice was investigated. Secondly, the use of 67Cu-labeled Lym-1 was evaluated in patients. In nude mice implanted with Raji human lymphoma, a greater than 2-fold enhancement of tumor uptake of 125I-Lym-1 was observed after administration of PEG-interleukin-2 (PEG-IL-2). The macrocycle 1,4,8,11-tetraazacylcotetradecane-N,N′,N′,N'''-tetraacetic acid (TETA), synthesized specifically for copper chelation, has been conjugated to Lym-1 for 67Cu labeling of the monoclonal antibody (MoAb). There was no evidence for bone or normal marrow uptake and the residence time in the tumor was prolonged. Surprisingly, a dose of 4.4 mCi that was intended for imaging induced substantial tumor regression in a patient.
| Original language | English (US) |
|---|---|
| Journal | International Journal of Radiation Applications and Instrumentation. |
| Volume | 18 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1991 |
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Strategies for enhancement of radioimmunotherapy. / Denardo, Gerald L; DeNardo, S.; Kukis, D.; Diril, H.; Suey, C.; Meares, Claude.
In: International Journal of Radiation Applications and Instrumentation., Vol. 18, No. 6, 1991.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Strategies for enhancement of radioimmunotherapy
AU - Denardo, Gerald L
AU - DeNardo, S.
AU - Kukis, D.
AU - Diril, H.
AU - Suey, C.
AU - Meares, Claude
PY - 1991
Y1 - 1991
N2 - Two new strategies for increasing tumor uptake have been investigated. First the effect of interleukin-2 (IL-2) on tumor uptake of 125I-Lym-l antibody in nude mice was investigated. Secondly, the use of 67Cu-labeled Lym-1 was evaluated in patients. In nude mice implanted with Raji human lymphoma, a greater than 2-fold enhancement of tumor uptake of 125I-Lym-1 was observed after administration of PEG-interleukin-2 (PEG-IL-2). The macrocycle 1,4,8,11-tetraazacylcotetradecane-N,N′,N′,N'''-tetraacetic acid (TETA), synthesized specifically for copper chelation, has been conjugated to Lym-1 for 67Cu labeling of the monoclonal antibody (MoAb). There was no evidence for bone or normal marrow uptake and the residence time in the tumor was prolonged. Surprisingly, a dose of 4.4 mCi that was intended for imaging induced substantial tumor regression in a patient.
AB - Two new strategies for increasing tumor uptake have been investigated. First the effect of interleukin-2 (IL-2) on tumor uptake of 125I-Lym-l antibody in nude mice was investigated. Secondly, the use of 67Cu-labeled Lym-1 was evaluated in patients. In nude mice implanted with Raji human lymphoma, a greater than 2-fold enhancement of tumor uptake of 125I-Lym-1 was observed after administration of PEG-interleukin-2 (PEG-IL-2). The macrocycle 1,4,8,11-tetraazacylcotetradecane-N,N′,N′,N'''-tetraacetic acid (TETA), synthesized specifically for copper chelation, has been conjugated to Lym-1 for 67Cu labeling of the monoclonal antibody (MoAb). There was no evidence for bone or normal marrow uptake and the residence time in the tumor was prolonged. Surprisingly, a dose of 4.4 mCi that was intended for imaging induced substantial tumor regression in a patient.
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UR - http://www.scopus.com/inward/citedby.url?scp=44949281125&partnerID=8YFLogxK
U2 - 10.1016/0883-2897(91)90033-H
DO - 10.1016/0883-2897(91)90033-H
M3 - Article
C2 - 1743986
AN - SCOPUS:44949281125
VL - 18
JO - Nuclear Medicine and Biology
JF - Nuclear Medicine and Biology
SN - 0969-8051
IS - 6
ER -