Store-operated Ca2+ entry: Evidence for a secretion-like coupling model

R. L. Patterson, D. B. Van Rossum, D. L. Gill

Research output: Contribution to journalArticlepeer-review

385 Scopus citations


The elusive coupling between endoplasmic reticulum (ER) Ca2+ stores and plasma membrane (PM) 'storeoperated' Ca2+ entry channels was probed through a novel combination of cytoskeletal modifications. Whereas coupling was unaffected by disassembly of the actin cytoskeleton, in situ redistribution of F-actin into a tight cortical layer subjacent to the PM displaced cortical ER and prevented coupling between ER and PM Ca2+ entry channels, while not affecting inositol 1,4,5-trisphosphate-mediated store release. Importantly, disassembly of the induced cortical actin layer allowed ER to regain access to the PM and reestablish coupling of Ca2+ entry channels to Ca2+ store depletion. Coupling is concluded to be mediated by a physical 'secretion-like' mechanism involving close but reversible interactions between the ER and the PM.

Original languageEnglish (US)
Pages (from-to)487-499
Number of pages13
Issue number4
StatePublished - Aug 20 1999
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology


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