Store-operated Ca2+ entry

Evidence for a secretion-like coupling model

R. L. Patterson, D. B. Van Rossum, D. L. Gill

Research output: Contribution to journalArticle

373 Citations (Scopus)

Abstract

The elusive coupling between endoplasmic reticulum (ER) Ca2+ stores and plasma membrane (PM) 'storeoperated' Ca2+ entry channels was probed through a novel combination of cytoskeletal modifications. Whereas coupling was unaffected by disassembly of the actin cytoskeleton, in situ redistribution of F-actin into a tight cortical layer subjacent to the PM displaced cortical ER and prevented coupling between ER and PM Ca2+ entry channels, while not affecting inositol 1,4,5-trisphosphate-mediated store release. Importantly, disassembly of the induced cortical actin layer allowed ER to regain access to the PM and reestablish coupling of Ca2+ entry channels to Ca2+ store depletion. Coupling is concluded to be mediated by a physical 'secretion-like' mechanism involving close but reversible interactions between the ER and the PM.

Original languageEnglish (US)
Pages (from-to)487-499
Number of pages13
JournalCell
Volume98
Issue number4
DOIs
StatePublished - Aug 20 1999
Externally publishedYes

Fingerprint

Cell membranes
Endoplasmic Reticulum
Cell Membrane
Actins
Regain
Inositol 1,4,5-Trisphosphate
Actin Cytoskeleton

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Store-operated Ca2+ entry : Evidence for a secretion-like coupling model. / Patterson, R. L.; Van Rossum, D. B.; Gill, D. L.

In: Cell, Vol. 98, No. 4, 20.08.1999, p. 487-499.

Research output: Contribution to journalArticle

Patterson, RL, Van Rossum, DB & Gill, DL 1999, 'Store-operated Ca2+ entry: Evidence for a secretion-like coupling model', Cell, vol. 98, no. 4, pp. 487-499. https://doi.org/10.1016/S0092-8674(00)81977-7
Patterson, R. L. ; Van Rossum, D. B. ; Gill, D. L. / Store-operated Ca2+ entry : Evidence for a secretion-like coupling model. In: Cell. 1999 ; Vol. 98, No. 4. pp. 487-499.
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