Stoichiometry of reconstituted high-density lipoproteins in the hydrated state determined by photon antibunching

Sonny Ly, Jitka Petrlova, Thomas R Huser, Samantha Fore, Tingjuan Gao, John C Voss, Ted A. Laurence

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Apolipoprotein A-I plays a central role in the solution structure of high-density lipoproteins. Determining the stoichiometry of lipid-bound apo A-I in the hydrated state is therefore fundamental to understanding how high-density lipoproteins form and function. Here, we use the quantum optical phenomenon of photon antibunching to determine the number of apo A-I molecules bound to discoidal lipoproteins and compare this with values obtained by photon-counting histogram analysis. Both the photon antibunching and photon-counting analyses show that reconstituted high-density lipoprotein particles contain two apo A-I molecules, which is in agreement with the commonly accepted double-belt model.

Original languageEnglish (US)
Pages (from-to)970-975
Number of pages6
JournalBiophysical Journal
Volume101
Issue number4
DOIs
StatePublished - Aug 17 2011

ASJC Scopus subject areas

  • Biophysics

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