Stochastic simulation of actin dynamics reveals the role of annealing and fragmentation

Joseph Fass, Chi Pak, James Bamburg, Alex Mogilner

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Recent observations of F-actin dynamics call for theoretical models to interpret and understand the quantitative data. A number of existing models rely on simplifications and do not take into account F-actin fragmentation and annealing. We use Gillespie's algorithm for stochastic simulations of the F-actin dynamics including fragmentation and annealing. The simulations vividly illustrate that fragmentation and annealing have little influence on the shape of the polymerization curve and on nucleotide profiles within filaments but drastically affect the F-actin length distribution, making it exponential. We find that recent surprising measurements of high length diffusivity at the critical concentration cannot be explained by fragmentation and annealing events unless both fragmentation rates and frequency of undetected fragmentation and annealing events are greater than previously thought. The simulations compare well with experimentally measured actin polymerization data and lend additional support to a number of existing theoretical models.

Original languageEnglish (US)
Pages (from-to)173-183
Number of pages11
JournalJournal of Theoretical Biology
Volume252
Issue number1
DOIs
StatePublished - May 7 2008

Fingerprint

annealing
Actin
Stochastic Simulation
Fragmentation
Annealing
actin
Actins
Polymerization
polymerization
Theoretical Models
Theoretical Model
Nucleotides
diffusivity
Filament
Diffusivity
Simplification
nucleotides
Simulation
Curve

Keywords

  • Cytoskeleton
  • Monte Carlo simulations
  • Polymer length

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Stochastic simulation of actin dynamics reveals the role of annealing and fragmentation. / Fass, Joseph; Pak, Chi; Bamburg, James; Mogilner, Alex.

In: Journal of Theoretical Biology, Vol. 252, No. 1, 07.05.2008, p. 173-183.

Research output: Contribution to journalArticle

Fass, Joseph ; Pak, Chi ; Bamburg, James ; Mogilner, Alex. / Stochastic simulation of actin dynamics reveals the role of annealing and fragmentation. In: Journal of Theoretical Biology. 2008 ; Vol. 252, No. 1. pp. 173-183.
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