Stimulatory effect of mevinolin on rat liver phosphatidic acid phosphatase

Ingemar Björkhem, Lars Berglund

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The activity of the soluble form of phosphatidic acid phosphatase in rat liver was stimulated about 2.5-fold by inclusion of mevinolin, a competitive hydroxymethylglutaryl-CoA reductase inhibitor, in the diet (0.1%). The stimulatory effect of mevinolin was present also after dietary addition of cholestyramine (5%) or intraperitoneal administration of ethanol. Addition of cholesterol (2%) to the diet totally abolished the stimulation by mevinolin on phosphatidic acid phosphatase. The results support a correlation between the synthesis of the rate-limiting enzyme in cholesterol biosynthesis and the activity of the apparent rate-limiting enzyme in triacylglycerol biosynthesis.

Original languageEnglish (US)
Pages (from-to)20-25
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume920
Issue number1
DOIs
StatePublished - Jul 13 1987
Externally publishedYes

Fingerprint

Phosphatidate Phosphatase
Lovastatin
Liver
Rats
Biosynthesis
Nutrition
Cholesterol
Diet
Cholestyramine Resin
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Enzymes
Triglycerides
Ethanol

Keywords

  • (Rat liver)
  • Cholesterol synthesis
  • Hydroxymethylglutaryl-CoA reductase
  • Phosphatidic acid phosphatase
  • Triacylglycerol synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

Stimulatory effect of mevinolin on rat liver phosphatidic acid phosphatase. / Björkhem, Ingemar; Berglund, Lars.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 920, No. 1, 13.07.1987, p. 20-25.

Research output: Contribution to journalArticle

@article{09a4387f85624eea993c640a3c26d9a3,
title = "Stimulatory effect of mevinolin on rat liver phosphatidic acid phosphatase",
abstract = "The activity of the soluble form of phosphatidic acid phosphatase in rat liver was stimulated about 2.5-fold by inclusion of mevinolin, a competitive hydroxymethylglutaryl-CoA reductase inhibitor, in the diet (0.1{\%}). The stimulatory effect of mevinolin was present also after dietary addition of cholestyramine (5{\%}) or intraperitoneal administration of ethanol. Addition of cholesterol (2{\%}) to the diet totally abolished the stimulation by mevinolin on phosphatidic acid phosphatase. The results support a correlation between the synthesis of the rate-limiting enzyme in cholesterol biosynthesis and the activity of the apparent rate-limiting enzyme in triacylglycerol biosynthesis.",
keywords = "(Rat liver), Cholesterol synthesis, Hydroxymethylglutaryl-CoA reductase, Phosphatidic acid phosphatase, Triacylglycerol synthesis",
author = "Ingemar Bj{\"o}rkhem and Lars Berglund",
year = "1987",
month = "7",
day = "13",
doi = "10.1016/0005-2760(87)90306-7",
language = "English (US)",
volume = "920",
pages = "20--25",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Stimulatory effect of mevinolin on rat liver phosphatidic acid phosphatase

AU - Björkhem, Ingemar

AU - Berglund, Lars

PY - 1987/7/13

Y1 - 1987/7/13

N2 - The activity of the soluble form of phosphatidic acid phosphatase in rat liver was stimulated about 2.5-fold by inclusion of mevinolin, a competitive hydroxymethylglutaryl-CoA reductase inhibitor, in the diet (0.1%). The stimulatory effect of mevinolin was present also after dietary addition of cholestyramine (5%) or intraperitoneal administration of ethanol. Addition of cholesterol (2%) to the diet totally abolished the stimulation by mevinolin on phosphatidic acid phosphatase. The results support a correlation between the synthesis of the rate-limiting enzyme in cholesterol biosynthesis and the activity of the apparent rate-limiting enzyme in triacylglycerol biosynthesis.

AB - The activity of the soluble form of phosphatidic acid phosphatase in rat liver was stimulated about 2.5-fold by inclusion of mevinolin, a competitive hydroxymethylglutaryl-CoA reductase inhibitor, in the diet (0.1%). The stimulatory effect of mevinolin was present also after dietary addition of cholestyramine (5%) or intraperitoneal administration of ethanol. Addition of cholesterol (2%) to the diet totally abolished the stimulation by mevinolin on phosphatidic acid phosphatase. The results support a correlation between the synthesis of the rate-limiting enzyme in cholesterol biosynthesis and the activity of the apparent rate-limiting enzyme in triacylglycerol biosynthesis.

KW - (Rat liver)

KW - Cholesterol synthesis

KW - Hydroxymethylglutaryl-CoA reductase

KW - Phosphatidic acid phosphatase

KW - Triacylglycerol synthesis

UR - http://www.scopus.com/inward/record.url?scp=0023187887&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023187887&partnerID=8YFLogxK

U2 - 10.1016/0005-2760(87)90306-7

DO - 10.1016/0005-2760(87)90306-7

M3 - Article

VL - 920

SP - 20

EP - 25

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 1

ER -