Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model

Woo Sung Jung, Sei Myoung Han, Sung Min Kim, Mi Eun Kim, Jun Sik Lee, Kyoung Won Seo, Hwa Young Youn, Hee Woo Lee

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The thymus is the central lymphoid organ providing a unique and essential microenvironment for T-cell precursor development into mature functionally competent T-lymphocytes. Thus, it is important to develop the strategies for enhancing thymic regeneration from involution induced by a variety of clinical treatments and conditions. Hepatocyte growth factor (HGF) promotes proliferation in a variety of cell types. We have used stem cell-based HGF gene therapy to enhance regeneration from acute thymic involution. HGF-overexpressing human adipose tissue-derived mesenchymal stem cells (HGF-hATMSCs) were generated by liposomal transfection with the pMEX expression vector, constructed by inserting the HGF gene. Significantly increased HGF expression in these cells was confirmed by reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay. HGF produced by HGF-hATMSCs enhanced the proliferation of a mouse thymic epithelial cell line and the expression of interleukin-7 in vitro. We also examined the effect of HGF-hATMSCs on thymic regeneration in rats with acute thymic involution. Significant increases in thymus size and weight, as well as the number of thymocytes (especially, early thymocyte progenitors), were seen in the HGF-hATMSCs-treated rats compared to saline-treated control animals. A stimulatory effect of HGF-hATMSCs on thymic regeneration has therefore been shown, highlighting the clinical value of HGF-hATMSCs for treating thymic involution.

Original languageEnglish (US)
Pages (from-to)1106-1117
Number of pages12
JournalCell Biology International
Volume38
Issue number10
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Keywords

  • Adipose tissue-derived mesenchymal stem cells
  • Gene therapy
  • Hepatocyte growth factor
  • Regeneration
  • Thymocytes
  • Thymus

ASJC Scopus subject areas

  • Cell Biology

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