Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model

Woo Sung Jung, Sei Myoung Han, Sung Min Kim, Mi Eun Kim, Jun Sik Lee, Kyoung Won Seo, Hwa Young Youn, Hee Woo Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The thymus is the central lymphoid organ providing a unique and essential microenvironment for T-cell precursor development into mature functionally competent T-lymphocytes. Thus, it is important to develop the strategies for enhancing thymic regeneration from involution induced by a variety of clinical treatments and conditions. Hepatocyte growth factor (HGF) promotes proliferation in a variety of cell types. We have used stem cell-based HGF gene therapy to enhance regeneration from acute thymic involution. HGF-overexpressing human adipose tissue-derived mesenchymal stem cells (HGF-hATMSCs) were generated by liposomal transfection with the pMEX expression vector, constructed by inserting the HGF gene. Significantly increased HGF expression in these cells was confirmed by reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay. HGF produced by HGF-hATMSCs enhanced the proliferation of a mouse thymic epithelial cell line and the expression of interleukin-7 in vitro. We also examined the effect of HGF-hATMSCs on thymic regeneration in rats with acute thymic involution. Significant increases in thymus size and weight, as well as the number of thymocytes (especially, early thymocyte progenitors), were seen in the HGF-hATMSCs-treated rats compared to saline-treated control animals. A stimulatory effect of HGF-hATMSCs on thymic regeneration has therefore been shown, highlighting the clinical value of HGF-hATMSCs for treating thymic involution.

Original languageEnglish (US)
Pages (from-to)1106-1117
Number of pages12
JournalCell Biology International
Volume38
Issue number10
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Hepatocyte Growth Factor
Mesenchymal Stromal Cells
Thymus Gland
Adipose Tissue
Regeneration
Thymocytes
T-Lymphoid Precursor Cells
Interleukin-7
Genetic Therapy
Reverse Transcription
Transfection
Stem Cells
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Weights and Measures
Cell Line
Polymerase Chain Reaction

Keywords

  • Adipose tissue-derived mesenchymal stem cells
  • Gene therapy
  • Hepatocyte growth factor
  • Regeneration
  • Thymocytes
  • Thymus

ASJC Scopus subject areas

  • Cell Biology

Cite this

Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model. / Jung, Woo Sung; Han, Sei Myoung; Kim, Sung Min; Kim, Mi Eun; Lee, Jun Sik; Seo, Kyoung Won; Youn, Hwa Young; Lee, Hee Woo.

In: Cell Biology International, Vol. 38, No. 10, 01.01.2014, p. 1106-1117.

Research output: Contribution to journalArticle

Jung, Woo Sung ; Han, Sei Myoung ; Kim, Sung Min ; Kim, Mi Eun ; Lee, Jun Sik ; Seo, Kyoung Won ; Youn, Hwa Young ; Lee, Hee Woo. / Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model. In: Cell Biology International. 2014 ; Vol. 38, No. 10. pp. 1106-1117.
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