Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families

Céline Robert-Tissot, Vera L. Rüegger, Valentino Cattori, Marina L. Meli, Barbara Riond, Peter F Moore, Monika Engels, Marco Franchini, Regina Hofmann-Lehmann, Hans Lutz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Domestic cats are commonly affected by viral pathogens that induce lengthy infections with fatal outcomes. Prevention of viral propagation is of primordial importance in shelters and catteries, where cats from different backgrounds have narrow contacts. Oligonucleotides (ODN) containing cytosine-phosphate- guanosine motifs of class A (CpG-A) are highly potent synthetic inducers of innate antiviral mechanisms. The aim of this study was to test their ability to modulate innate immune responses and prevent viral replication as stand-alone agents in the domestic cat. CpG-A stimulation of feline peripheral blood mononuclear cells (PBMCs) enhanced their proliferation, increased the presence of co-stimulatory molecules on their surface and influenced their gene expression profiles in an antiviral orientation. Incubation of the supernatants of CpG-A stimulated PBMCs with feline cell lines of epithelial and fibroblastic origin induced expression of the antiviral myxovirus resistance (Mx) gene in these target cells, which also showed enhanced resistance to feline viruses from five distinct families, namely Coronaviridae, Herpesviridae, Caliciviridae, Parvoviridae, and Retroviridae. Most importantly, subcutaneous administration of CpG-A in domestic cats systemically increased the expression of Mx, reaching maximal levels within 24 h. Plasma from treated cats could furthermore inhibit viral replication in vitro. Altogether, our data highlight the promising potential of CpG-A to induce a preventive antiviral state in the cat and to protect feline populations against a broad range of virus infections.

Original languageEnglish (US)
Article number60
JournalVeterinary Research
Volume43
Issue number1
DOIs
StatePublished - 2012

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oligodeoxyribonucleotides
Felidae
Oligonucleotides
Cats
cats
Viruses
viruses
Antiviral Agents
Infection
infection
Coronaviridae
Parvoviridae
Blood Cells
Caliciviridae
mononuclear leukocytes
virus replication
Guanine Nucleotides
Fatal Outcome
Herpesviridae
Cytosine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families. / Robert-Tissot, Céline; Rüegger, Vera L.; Cattori, Valentino; Meli, Marina L.; Riond, Barbara; Moore, Peter F; Engels, Monika; Franchini, Marco; Hofmann-Lehmann, Regina; Lutz, Hans.

In: Veterinary Research, Vol. 43, No. 1, 60, 2012.

Research output: Contribution to journalArticle

Robert-Tissot, C, Rüegger, VL, Cattori, V, Meli, ML, Riond, B, Moore, PF, Engels, M, Franchini, M, Hofmann-Lehmann, R & Lutz, H 2012, 'Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families', Veterinary Research, vol. 43, no. 1, 60. https://doi.org/10.1186/1297-9716-43-60
Robert-Tissot, Céline ; Rüegger, Vera L. ; Cattori, Valentino ; Meli, Marina L. ; Riond, Barbara ; Moore, Peter F ; Engels, Monika ; Franchini, Marco ; Hofmann-Lehmann, Regina ; Lutz, Hans. / Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families. In: Veterinary Research. 2012 ; Vol. 43, No. 1.
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