Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin

S. Vukicevic, F. P. Luyten, A Hari Reddi

Research output: Contribution to journalArticle

201 Scopus citations

Abstract

Osteogenin was recently purified and the amino acid sequences of tryptic peptides were determined. Osteogenin in conjunction with insoluble collagenous bone matrix induces cartilage and bone formation in vivo. To understnd the mechanism of action of osteogenin, we examined its influence on periosteal cells, osteoblasts, fibroblasts, chondrocytes, and bone marrow stromal cells in vitro. Osteogenin stimulated alkaline phosphatase activity and collagen synthesis in periosteal cells. The cAMP response to parathyroid hormone in periosteal cells was increased by osteogenin. In primary cultures of calvarial osteoblasts, osteogenin stimulated alkaline phosphatase activity, the cAMP response to parathyroid hormone, and the synthesis of collagenous and noncollagenous proteins; however, cell proliferation was not affected. Osteogenin increased the production of sulfated proteoglycans in fetal rat chondroblasts and in rabbit articular chondrocytes. The present experiments demonstrate the significant influence of osteogenin in the stimulation of osteogenic and chondrogenic phenotypes in vitro.

Original languageEnglish (US)
Pages (from-to)8793-8797
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number22
DOIs
StatePublished - 1989
Externally publishedYes

Keywords

  • alkaline phosphatase
  • bone marrow stroma
  • chondrocytes
  • osteoblasts
  • periosteal cells

ASJC Scopus subject areas

  • Genetics
  • General

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