Stimulation of the CD2 receptor pathway induces apoptosis in human T lymphotropic virus type I-infected cell lines

Deborah J. Guyot; O. Joseph Trask; Janice M. Andrews; Garret C. Newbound; Michael Dale Lairmore

Stimulation of the CD2 receptor pathway induces apoptosis in human T lymphotropic virus type I-infected cell lines

Deborah J. Guyot, O. Joseph Trask, Janice M. Andrews, Garret C. Newbound, Michael Dale Lairmore

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate that CD2 receptor engagement, but not CD3 cross-linking, induces apoptosis in lymphocytes transformed by human T-cell lymphotropic virus type I (HTLV-I). Mitogenic pairs of anti-CD2 monoclonal antibodies inhibited [³H]thymidine incorporation from 25 to 62% in CD2⁺ HTLV-I- infected lymphocytes. This inhibition was associated with a 20-40% reduction in cell number and viability over a 3-day period, morphologic evidence of apoptosis, and irreversible DNA fragmentation. While cyclosporin A abrogated CD2-mediated proliferation in peripheral blood mononuclear cells, it had no effect on CD2-induced apoptosis in the HTLV-I-infected cell lines. Since HTLV-I is mitogenic to resting lymphocytes through CD2 activation pathways, these results suggest that HTLV-I-infected lymphocytes are primed for apoptosis following additional CD2 stimulation. This CD2-mediated apoptosis might be a factor in immune regulation of HTLV-I-associated diseases or might offer a novel adjunctive approach to treatment.

Original language	English (US)
Pages (from-to)	317-325
Number of pages	9
Journal	Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume	11
Issue number	4
State	Published - 1996
Externally published	Yes

Keywords

Apoptosis
CD2
CD3
Human T lymphotropic virus type I

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Virology

Fingerprint Dive into the research topics of 'Stimulation of the CD2 receptor pathway induces apoptosis in human T lymphotropic virus type I-infected cell lines'. Together they form a unique fingerprint.

Cite this

Stimulation of the CD2 receptor pathway induces apoptosis in human T lymphotropic virus type I-infected cell lines. / Guyot, Deborah J.; Joseph Trask, O.; Andrews, Janice M.; Newbound, Garret C.; Lairmore, Michael Dale.

In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 11, No. 4, 1996, p. 317-325.

Research output: Contribution to journal › Article › peer-review

@article{0b628a40e09244e98235924918f4d6cc,

title = "Stimulation of the CD2 receptor pathway induces apoptosis in human T lymphotropic virus type I-infected cell lines",

abstract = "We demonstrate that CD2 receptor engagement, but not CD3 cross-linking, induces apoptosis in lymphocytes transformed by human T-cell lymphotropic virus type I (HTLV-I). Mitogenic pairs of anti-CD2 monoclonal antibodies inhibited [3H]thymidine incorporation from 25 to 62% in CD2+ HTLV-I- infected lymphocytes. This inhibition was associated with a 20-40% reduction in cell number and viability over a 3-day period, morphologic evidence of apoptosis, and irreversible DNA fragmentation. While cyclosporin A abrogated CD2-mediated proliferation in peripheral blood mononuclear cells, it had no effect on CD2-induced apoptosis in the HTLV-I-infected cell lines. Since HTLV-I is mitogenic to resting lymphocytes through CD2 activation pathways, these results suggest that HTLV-I-infected lymphocytes are primed for apoptosis following additional CD2 stimulation. This CD2-mediated apoptosis might be a factor in immune regulation of HTLV-I-associated diseases or might offer a novel adjunctive approach to treatment.",

keywords = "Apoptosis, CD2, CD3, Human T lymphotropic virus type I",

author = "Guyot, {Deborah J.} and {Joseph Trask}, O. and Andrews, {Janice M.} and Newbound, {Garret C.} and Lairmore, {Michael Dale}",

year = "1996",

language = "English (US)",

volume = "11",

pages = "317--325",

journal = "Journal of acquired immune deficiency syndromes (1999)",

issn = "1525-4135",

publisher = "Lippincott Williams and Wilkins",

number = "4",

}

TY - JOUR

T1 - Stimulation of the CD2 receptor pathway induces apoptosis in human T lymphotropic virus type I-infected cell lines

AU - Guyot, Deborah J.

AU - Joseph Trask, O.

AU - Andrews, Janice M.

AU - Newbound, Garret C.

AU - Lairmore, Michael Dale

PY - 1996

Y1 - 1996

N2 - We demonstrate that CD2 receptor engagement, but not CD3 cross-linking, induces apoptosis in lymphocytes transformed by human T-cell lymphotropic virus type I (HTLV-I). Mitogenic pairs of anti-CD2 monoclonal antibodies inhibited [3H]thymidine incorporation from 25 to 62% in CD2+ HTLV-I- infected lymphocytes. This inhibition was associated with a 20-40% reduction in cell number and viability over a 3-day period, morphologic evidence of apoptosis, and irreversible DNA fragmentation. While cyclosporin A abrogated CD2-mediated proliferation in peripheral blood mononuclear cells, it had no effect on CD2-induced apoptosis in the HTLV-I-infected cell lines. Since HTLV-I is mitogenic to resting lymphocytes through CD2 activation pathways, these results suggest that HTLV-I-infected lymphocytes are primed for apoptosis following additional CD2 stimulation. This CD2-mediated apoptosis might be a factor in immune regulation of HTLV-I-associated diseases or might offer a novel adjunctive approach to treatment.

AB - We demonstrate that CD2 receptor engagement, but not CD3 cross-linking, induces apoptosis in lymphocytes transformed by human T-cell lymphotropic virus type I (HTLV-I). Mitogenic pairs of anti-CD2 monoclonal antibodies inhibited [3H]thymidine incorporation from 25 to 62% in CD2+ HTLV-I- infected lymphocytes. This inhibition was associated with a 20-40% reduction in cell number and viability over a 3-day period, morphologic evidence of apoptosis, and irreversible DNA fragmentation. While cyclosporin A abrogated CD2-mediated proliferation in peripheral blood mononuclear cells, it had no effect on CD2-induced apoptosis in the HTLV-I-infected cell lines. Since HTLV-I is mitogenic to resting lymphocytes through CD2 activation pathways, these results suggest that HTLV-I-infected lymphocytes are primed for apoptosis following additional CD2 stimulation. This CD2-mediated apoptosis might be a factor in immune regulation of HTLV-I-associated diseases or might offer a novel adjunctive approach to treatment.

KW - Apoptosis

KW - CD2

KW - CD3

KW - Human T lymphotropic virus type I

UR - http://www.scopus.com/inward/record.url?scp=9244239221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9244239221&partnerID=8YFLogxK

M3 - Article

C2 - 8601217

AN - SCOPUS:9244239221

VL - 11

SP - 317

EP - 325

JO - Journal of acquired immune deficiency syndromes (1999)

JF - Journal of acquired immune deficiency syndromes (1999)

SN - 1525-4135

IS - 4

ER -